Raji Whole Cell Lysate: sc-364236
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; Burkitt's lymphoma B lymphocyte cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
QUICK LINKS
SEE ALSO...
Raji Whole Cell Lysate is derived from the Raji cell line, an established human B-cell line from Burkitt's lymphoma, widely utilized in immunological and biochemical research. This lysate contains a comprehensive profile of cellular proteins, enabling the study of cellular mechanisms and signaling pathways relevant to B-cell biology and lymphoma. Researchers use the Raji Whole Cell Lysate primarily to examine cellular responses to various stimuli and the interaction of viral proteins with cellular machinery, given the line's origin and susceptibility to viral infections. It serves as an essential tool for studying the Epstein-Barr virus (EBV) interactions, as Raji cells contain EBV genomes, making them a model system for understanding EBV-related oncogenesis and immune evasion strategies. The lysate is instrumental in dissecting pathways like NF-κB, which plays a crucial role in cell survival and proliferation. The use of this lysate in proteomic studies allows for the identification and quantification of proteins that are differentially expressed in cancerous conditions, aiding in the fundamental understanding of tumor biology and immune response.
Raji Whole Cell Lysate References:
- High-throughput detection of nuclear factor-kappaB activity using a sensitive oligo-based chemiluminescent enzyme-linked immunosorbent assay. | Bhattacharya, N., et al. 2010. Int J Cancer. 127: 404-11. PMID: 19924814
- Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent. | Kyathanahalli, C., et al. 2015. Proc Natl Acad Sci U S A. 112: 14090-5. PMID: 26504199
- Automated Ligand Purification Platform Accelerates Immunopeptidome Analysis by Mass Spectrometry. | Zhang, L., et al. 2021. J Proteome Res. 20: 393-408. PMID: 33331781
- Human Ia beta chains and the invariant chain share a common antigenic determinant. | Finn, OJ., et al. 1983. J Exp Med. 158: 1344-9. PMID: 6194245
- Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade. | Hu, MC., et al. 1996. Genes Dev. 10: 2251-64. PMID: 8824585
- Regulatory factor X, a bare lymphocyte syndrome transcription factor, is a multimeric phosphoprotein complex. | Moreno, CS., et al. 1997. J Immunol. 158: 5841-8. PMID: 9190936
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Raji Whole Cell Lysate | sc-364236 | 500 µg/200 µl | $118.00 |