Jurkat + pervanadate Cell Lysate: sc-24716

Jurkat + Pervanadate Cell Lysate is derived from Jurkat cells, a human T-cell leukemia line, after treatment with pervanadate, a potent oxidizing agent that acts as a tyrosine phosphatase inhibitor. This specific treatment is utilized to mimic cellular conditions that lead to increased tyrosine phosphorylation, a critical post-translational modification involved in signaling pathways related to cell growth, differentiation, and survival. The lysate itself is a complex mixture of proteins, nucleic acids, and other cellular components that encapsulate the state of the cell following pervanadate exposure, providing a snapshot of cellular processes influenced by inhibited phosphatase activity. Researchers employ this lysate in various studies to explore the impact of enhanced tyrosine phosphorylation on cell signaling networks. It is particularly valuable for investigating the pathways that lead to activation or inhibition of apoptosis, as well as those involved in the activation of transcription factors and other signaling molecules. By analyzing Jurkat + Pervanadate Cell Lysate, scientists can gain insights into the regulatory mechanisms that control T-cell behavior, contribute to signal transduction research, and deepen our understanding of cellular responses to oxidative stress and enzymatic inhibition within the immune system.