WEHI-231 Whole Cell Lysate: sc-2213

WEHI-231 Whole Cell Lysate is derived from WEHI-231 cells, a murine B-cell lymphoma cell line originating from a BALB/c mouse. This lysate is extensively utilized in immunological and oncological research to study B-cell signaling, proliferation, and apoptosis. WEHI-231 cells are known for their sensitivity to B-cell receptor (BCR) signaling, making the lysate a valuable resource for investigating the molecular mechanisms underlying B-cell activation and tolerance. Researchers employ WEHI-231 Whole Cell Lysate to analyze the expression and post-translational modifications of key signaling proteins involved in the BCR pathway, such as SYK, BLNK, and BTK, using techniques like western blotting, immunoprecipitation, and mass spectrometry. This lysate is particularly useful for studying the interactions and activation states of these proteins and their downstream effectors, including the NF-κB and PI3K/AKT pathways, which are crucial for cell survival and proliferation. Additionally, WEHI-231 Whole Cell Lysate is used to screen for chemical inhibitors and activators that modulate B-cell signaling, providing insights into the cellular processes that contribute to lymphomagenesis and immune regulation. By comparing the lysates from WEHI-231 cells with those from normal B cells, scientists can explain the specific molecular alterations associated with B-cell lymphoma, advancing research in cellular signaling and cancer biology.