MDA-MB-231 Cell Lysate: sc-2232
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; breast adenocarcinoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
QUICK LINKS
SEE ALSO...
MDA-MB-231 cell lysate is derived from a highly aggressive and metastatic human breast cancer cell line, widely used in scientific research to study the mechanisms of cancer metastasis and invasion. This lysate serves as a vital tool for investigating the expression and regulatory mechanisms of genes and proteins involved in cell motility and epithelial-mesenchymal transition (EMT), a process pivotal for cancer cells to acquire invasive capabilities. Researchers utilize MDA-MB-231 lysate to study the roles of various signaling molecules and pathways, including the NF-kB pathway, which is known to be active in promoting inflammation and metastasis. Additionally, this lysate has been instrumental in examining the tumor microenvironment's influence on cancer progression, particularly how tumor cells interact with and modify their surroundings to facilitate metastasis. The lysate's utility extends to screening for potential anti-metastatic compounds, providing insights into the molecular underpinnings of resistance. By using MDA-MB-231 cell lysate, researchers can delve into the molecular biology of cancer metastasis, exploring how alterations in gene expression and protein function contribute to the complex biology of aggressive breast cancer.
MDA-MB-231 Cell Lysate References:
- Bone morphogenetic protein-2 blocks MDA MB 231 human breast cancer cell proliferation by inhibiting cyclin-dependent kinase-mediated retinoblastoma protein phosphorylation. | Ghosh-Choudhury, N., et al. 2000. Biochem Biophys Res Commun. 272: 705-11. PMID: 10860819
- ST6GalNAc I expression in MDA-MB-231 breast cancer cells greatly modifies their O-glycosylation pattern and enhances their tumourigenicity. | Julien, S., et al. 2006. Glycobiology. 16: 54-64. PMID: 16135558
- Distinct roles of nonmuscle myosin II isoforms in the regulation of MDA-MB-231 breast cancer cell spreading and migration. | Betapudi, V., et al. 2006. Cancer Res. 66: 4725-33. PMID: 16651425
- Procathepsin D expression correlates with invasive and metastatic phenotype of MDA-MB-231 derived cell lines. | Ohri, SS., et al. 2007. Int J Biol Macromol. 41: 204-9. PMID: 17397917
- Monocarboxylate transporter 4 regulates maturation and trafficking of CD147 to the plasma membrane in the metastatic breast cancer cell line MDA-MB-231. | Gallagher, SM., et al. 2007. Cancer Res. 67: 4182-9. PMID: 17483329
- Degradation of the tumor suppressor PML by Pin1 contributes to the cancer phenotype of breast cancer MDA-MB-231 cells. | Reineke, EL., et al. 2008. Mol Cell Biol. 28: 997-1006. PMID: 18039859
- Genistein induces cell apoptosis in MDA-MB-231 breast cancer cells via the mitogen-activated protein kinase pathway. | Li, Z., et al. 2008. Toxicol In Vitro. 22: 1749-53. PMID: 18761399
- Sphingosine kinase 2 prevents the nuclear translocation of sphingosine 1-phosphate receptor-2 and tyrosine 416 phosphorylated c-Src and increases estrogen receptor negative MDA-MB-231 breast cancer cell growth: The role of sphingosine 1-phosphate receptor-4. | Ohotski, J., et al. 2014. Cell Signal. 26: 1040-7. PMID: 24486401
- Diosgenin inhibits the migration of human breast cancer MDA-MB-231 cells by suppressing Vav2 activity. | He, Z., et al. 2014. Phytomedicine. 21: 871-6. PMID: 24656238
- Fluvastatin mediated breast cancer cell death: a proteomic approach to identify differentially regulated proteins in MDA-MB-231 cells. | Kanugula, AK., et al. 2014. PLoS One. 9: e108890. PMID: 25268751
- Effects of Lovastatin on MDA-MB-231 Breast Cancer Cells: An Antibody Microarray Analysis. | Yang, T., et al. 2016. J Cancer. 7: 192-9. PMID: 26819643
- Immunotherapy with dendritic cells and cytokine-induced killer cells for MDA-MB-231 breast cancer stem cells in nude mice. | Chen, Q., et al. 2016. Am J Transl Res. 8: 2947-55. PMID: 27508015
- Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1). | Endres, M., et al. 2016. Oncotarget. 7: 64244-64259. PMID: 27588391
- Epigallocatechin gallate inhibits the growth of MDA-MB-231 breast cancer cells via inactivation of the β-catenin signaling pathway. | Hong, OY., et al. 2017. Oncol Lett. 14: 441-446. PMID: 28693189
- Activity-Based Proteomic Identification of the S-Thiolation Targets of Ajoene in MDA-MB-231 Breast Cancer Cells. | Kusza, DA., et al. 2022. J Agric Food Chem. 70: 14679-14692. PMID: 36351177
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDA-MB-231 Cell Lysate | sc-2232 | 500 µg/200 µl | $118.00 |