NIH/3T3 + anisomycin Cell Lysate: sc-2247

NIH/3T3 + anisomycin cell lysate is derived from NIH/3T3 cells, a mouse embryonic fibroblast cell line, treated with anisomycin. Anisomycin is an antibiotic that inhibits protein synthesis by targeting the peptidyl transferase reaction on the ribosome. This lysate is extensively used in research to study stress-activated protein kinase (SAPK) pathways, particularly the JNK and p38 MAPK pathways. Anisomycin induces a robust activation of these pathways, leading to phosphorylation of downstream targets involved in stress responses, apoptosis, and inflammation. Researchers utilize NIH/3T3 + anisomycin cell lysate to investigate the molecular mechanisms underlying stress signaling and to identify key regulatory proteins involved in these pathways. This lysate facilitates the study of phosphorylation events and the regulation of transcription factors such as ATF2, c-Jun, and NF-κB, which are critical for gene expression in response to cellular stress. Additionally, it is used to examine the cross-talk between the JNK/p38 MAPK pathways and other signaling pathways, including the ERK and PI3K/Akt pathways. Recent studies have leveraged this lysate for proteomic and genomic analyses to identify novel signaling molecules and pathways activated by anisomycin. NIH/3T3 + anisomycin cell lysate continues to be a valuable resource for advancing our understanding of cellular stress responses and signaling mechanisms.