MCF7 + etopside Cell Lysate: sc-2281

MCF7 + etoposide cell lysate is derived from MCF7 cells, a human breast cancer cell line, treated with etoposide, a topoisomerase II inhibitor used to study the mechanisms of DNA damage and repair. This specific lysate facilitates a focused examination of the cellular response to DNA damage, particularly the activation of cell cycle checkpoints, apoptosis, and the pathways involved in genomic stability. Etoposide treatment induces DNA strand breaks, and the resulting lysate is crucial for investigating how cancer cells manage such damage. Researchers use this lysate to explore the activation of the p53 signaling pathway, which plays a key role in the cellular response to DNA damage by regulating cell cycle arrest and apoptosis. This is particularly relevant for understanding the molecular basis of chemosensitivity and resistance in cancer cells. The MCF7 + etoposide lysate is also used to assess the expression and activity of proteins involved in DNA repair mechanisms, such as RAD51, BRCA1, and other associated factors, providing insights into the intricacies of cellular repair systems. By studying these responses in a research context, the lysate aids in the understanding of how breast cancer cells react to chemotherapeutic agents, contributing to basic scientific knowledge on cancer cell biology and the molecular mechanisms of DNA damage response.