WEHI-3 Cell Lysate: sc-3815
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- mouse whole cell lysate; myelomonocyte cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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WEHI-3 Cell Lysate is derived from WEHI-3 cells, a murine myelomonocytic leukemia cell line established from a BALB/c mouse. This lysate is widely used in research to investigate the molecular mechanisms of leukemia and the differentiation of myeloid cells. WEHI-3 cells exhibit characteristics of immature myelomonocytes, making the lysate a valuable resource for studying hematopoiesis and leukemogenesis. Researchers utilize WEHI-3 Cell Lysate to analyze the expression and modification of proteins involved in cell cycle regulation, apoptosis, and signal transduction using techniques such as western blotting, immunoprecipitation, and proteomics. The lysate is particularly useful for examining the activation states of signaling pathways like JAK/STAT, PI3K/AKT, and MAPK/ERK, which play crucial roles in cell proliferation, survival, and differentiation. Additionally, WEHI-3 Cell Lysate is employed in screening chemical compounds to identify potential modulators of these pathways, providing insights into the cellular processes that contribute to leukemia progression. By comparing the lysates from WEHI-3 cells with those from normal myeloid cells, scientists can explain the specific molecular alterations associated with leukemia. This lysate is instrumental in advancing our understanding of the complex signaling networks and genetic aberrations driving myelomonocytic leukemia, aiding in the development of novel research strategies.
WEHI-3 Cell Lysate References:
- Specific binding of glucosaminylmuramyl peptides to histones. | Golovina, T., et al. 1999. FEBS Lett. 454: 152-6. PMID: 10413114
- Elucidating the inhibitory mechanisms of the ethanolic extract of the fruiting body of the mushroom Antrodia cinnamomea on the proliferation and migration of murine leukemia WEHI-3 cells and their tumorigenicity in a BALB/c allograft tumor model. | Liu, FC., et al. 2013. Phytomedicine. 20: 874-82. PMID: 23611488
- Macrophage surface glycoproteins binding to galectin-3 (Mac-2-antigen). | Dong, S. and Hughes, RC. 1997. Glycoconj J. 14: 267-74. PMID: 9111144
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WEHI-3 Cell Lysate | sc-3815 | 500 µg/200 µl | $118.00 |