SH-SY5Y Cell Lysate: sc-3812

SH-SY5Y cell lysate is derived from the SH-SY5Y cell line, a subclone of the SK-N-SH line, which itself originated from a human neuroblastoma. This lysate is extensively utilized in neuroscience research, particularly for studying neural differentiation and neurodegenerative disease mechanisms. SH-SY5Y cells are valued for their ability to differentiate into dopaminergic and cholinergic neurons under specific conditions, which makes their lysate a key tool in examining neuronal properties and responses to various biochemical stimuli. Researchers use SH-SY5Y lysate to investigate the cellular pathways involved in neuron function and survival, including the study of neurotrophic factors, neurotransmitter synthesis, and the signaling pathways related to Alzheimer's and Parkinson's diseases. The lysate facilitates a detailed analysis of protein interactions and post-translational modifications that occur during neuron differentiation and in response to neurotoxic stress. Additionally, it is employed to assess the impact of genetic modifications and environmental factors on neuronal viability and function. By providing insights into the molecular and cellular biology of neuroblastoma-derived neurons, SH-SY5Y cell lysate contributes significantly to the understanding of neuronal behavior.

SH-SY5Y Cell Lysate References:

1. Procaspase-3 and poly(ADP)ribose polymerase (PARP) are calpain substrates.  |  McGinnis, KM., et al. 1999. Biochem Biophys Res Commun. 263: 94-9. PMID: 10486259
2. Caspase-mediated proteolytic activation of calcineurin in thapsigargin-mediated apoptosis in SH-SY5Y neuroblastoma cells.  |  Mukerjee, N., et al. 2000. Arch Biochem Biophys. 379: 337-43. PMID: 10898953
3. Inhibition of mixed lineage kinase 3 attenuates MPP+-induced neurotoxicity in SH-SY5Y cells.  |  Mathiasen, JR., et al. 2004. Brain Res. 1003: 86-97. PMID: 15019567
4. A highly bioactive lignophenol derivative from bamboo lignin exhibits a potent activity to suppress apoptosis induced by oxidative stress in human neuroblastoma SH-SY5Y cells.  |  Akao, Y., et al. 2004. Bioorg Med Chem. 12: 4791-801. PMID: 15336257
5. Ischemia promotes calpain-mediated degradation of p120-catenin in SH-SY5Y cells.  |  Ohno, H., et al. 2007. Biochem Biophys Res Commun. 353: 547-52. PMID: 17196166
6. The expression of nicotinamide N-methyltransferase increases ATP synthesis and protects SH-SY5Y neuroblastoma cells against the toxicity of Complex I inhibitors.  |  Parsons, RB., et al. 2011. Biochem J. 436: 145-55. PMID: 21352099
7. Protective effect of orexin-A on 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y human dopaminergic neuroblastoma cells.  |  Esmaeili-Mahani, S., et al. 2013. Neurochem Int. 63: 719-25. PMID: 24135219
8. Glutathione adduct of methylmercury activates the Keap1-Nrf2 pathway in SH-SY5Y cells.  |  Yoshida, E., et al. 2014. Chem Res Toxicol. 27: 1780-6. PMID: 25271560
9. Expression of synaptophysin protein in different dopaminergic cell lines.  |  Bai, X. and Strong, R. 2014. J Biochem Pharmacol Res. 2: 185-190. PMID: 26279968
10. SPRi-MALDI MS: characterization and identification of a kinase from cell lysate by specific interaction with different designed ankyrin repeat proteins.  |  Anders, U., et al. 2017. Anal Bioanal Chem. 409: 1827-1836. PMID: 27987025
11. Rabbit Antidiethoxyphosphotyrosine Antibody, Made by Single B Cell Cloning, Detects Chlorpyrifos Oxon-Modified Proteins in Cultured Cells and Immunopurifies Modified Peptides for Mass Spectrometry.  |  Onder, S., et al. 2021. J Proteome Res. 20: 4728-4745. PMID: 34469172
12. Neuroprotective effects of Withania somnifera in the SH-SY5Y Parkinson cell model.  |  Wongtrakul, J., et al. 2021. Heliyon. 7: e08172. PMID: 34765761
13. Butyrylcholinesterase in SH-SY5Y human neuroblastoma cells.  |  Onder, S., et al. 2022. Neurotoxicology. 90: 1-9. PMID: 35189179