K-562 Whole Cell Lysate: sc-2203
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; chronic myelogenous leukemia cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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K-562 Whole Cell Lysate is derived from K-562 cells, an immortalized cell line originally established from a patient with chronic myelogenous leukemia (CML). These cells are of hematopoietic origin, specifically from the myeloid lineage, and are characterized by their undifferentiated, blast-like nature. The whole cell lysate encompasses a comprehensive profile of the cellular components, including proteins, nucleic acids, lipids, and metabolites, providing a snapshot of the cell's physiological state under normal culture conditions. This lysate is a valuable resource in research focused on hematological malignancies, allowing scientists to study basal cellular behaviors and properties in a leukemia model without the influence of external stimuli. It serves as a control in experiments where comparative analyses of genetic, proteomic, or biochemical responses to drugs, chemicals, or environmental changes are conducted. By utilizing K-562 Whole Cell Lysate, researchers can explore fundamental cellular processes such as cell cycle progression, signal transduction pathways, and gene expression in a cancerous context. This lysate is particularly useful for identifying targets for potential interventions, understanding disease mechanisms, and exploring the effects of genetic mutations inherent in CML cells. Through such studies, K-562 Whole Cell Lysate contributes significantly to basic cancer research and the broader field of cellular biology.
K-562 Whole Cell Lysate References:
- Presence of oxidized protein hydrolase in human cell lines, rat tissues, and human/rat plasma. | Fujino, T., et al. 2000. J Biochem. 127: 307-13. PMID: 10731698
- Human septin-septin interaction: CDCrel-1 partners with KIAA0202. | Bläser, S., et al. 2002. FEBS Lett. 519: 169-72. PMID: 12023038
- Tumor cell lysis by activated human neutrophils: analysis of neutrophil-delivered oxidative attack and role of leukocyte function-associated antigen 1. | Dallegri, F., et al. 1991. Inflammation. 15: 15-30. PMID: 1647368
- Erufosine: a membrane targeting antineoplastic agent with signal transduction modulating effects. | Zaharieva, MM., et al. 2007. Ann N Y Acad Sci. 1095: 182-92. PMID: 17404031
- Production and Transduction of a Human Recombinant β-Globin Chain into Proerythroid K-562 Cells To Replace Missing Endogenous β-Globin. | Papadopoulou, LC., et al. 2018. Mol Pharm. 15: 5665-5677. PMID: 30375878
- Human monocyte mediated cytotoxicity to K-562 cells: a freeze-fracture-, scanning- and thin section electron microscopic study. | Espevik, T., et al. 1982. Acta Pathol Microbiol Immunol Scand C. 90: 137-45. PMID: 7202321
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
K-562 Whole Cell Lysate | sc-2203 | 500 µg/200 µl | $118.00 |