NIH/3T3 + PMA Cell Lysate: sc-24748

NIH/3T3 + PMA cell lysate is derived from NIH/3T3 cells, a mouse embryonic fibroblast cell line, treated with phorbol 12-myristate 13-acetate (PMA). This lysate is extensively used in research to study the mechanisms of protein kinase C (PKC) activation and its downstream signaling pathways. PMA is a potent activator of PKC, which plays a crucial role in various cellular processes including differentiation, proliferation, and gene expression. Researchers use NIH/3T3 + PMA cell lysate to investigate the PKC-mediated signaling pathways and their effects on the mitogen-activated protein kinase (MAPK) pathways, such as ERK1/2, which are essential for cell growth and survival. This lysate facilitates the study of phosphorylation events and the regulation of transcription factors like AP-1 and NF-κB, which are critical for gene expression in response to external stimuli. Additionally, it is employed to examine the crosstalk between PKC and other signaling pathways, including the PI3K/Akt and JNK pathways. Recent studies have utilized NIH/3T3 + PMA cell lysate for proteomic and genomic analyses to identify novel substrates and signaling networks associated with PKC activation. By providing insights into the molecular mechanisms of PKC signaling, this lysate continues to be a valuable tool for advancing research in cell signaling and regulation.