RAW 264.7 + IFN-γ Cell Lysate: sc-2259

The RAW 264.7 + IFN-γ cell lysate is derived from the RAW 264.7 cell line, which originates from a murine macrophage cell type extensively employed in immunological and molecular biology research. This particular lysate is generated by treating RAW 264.7 cells with interferon-gamma (IFN-γ), a key cytokine involved in innate and adaptive immune responses. IFN-γ is a critical activator of the immune response, particularly in macrophages, where it upregulates the expression of major histocompatibility complex (MHC) class II molecules, enhancing antigen presentation capabilities. Additionally, IFN-γ activates several signal transducer and activator of transcription (STAT) pathways, primarily STAT1, leading to the transcription of genes involved in immune responsiveness, antiviral states, and cellular activation. This lysate is invaluable in research aimed at understanding macrophage activation, the role of cytokines in immune regulation, and the signaling mechanisms underlying macrophage responses to pathogens. By studying this lysate, researchers can explore the effects of IFN-γ on macrophage function, including cytokine production profiles, phagocytic activity, and intracellular signaling events, strictly within the context of basic scientific inquiry.