U266 Whole Cell Lysate: sc-364800

U266 is a B lymphocyte isolated from the peripheral blood of a 53-year-old, male patient with IgE-secreting myeloma. This lysate is widely used in cancer research to investigate the molecular mechanisms underlying multiple myeloma and other hematological malignancies. U266 cells are characterized by their high expression of immunoglobulin E (IgE) and the presence of specific genetic mutations, such as those affecting the MYC and KRAS genes. Researchers utilize U266 Whole Cell Lysate to analyze the expression, phosphorylation, and interaction of key proteins involved in signaling pathways critical for cell proliferation, survival, and apoptosis. Techniques such as western blotting, immunoprecipitation, and mass spectrometry are employed to study pathways including JAK/STAT, PI3K/AKT, and NF-κB, which play pivotal roles in myeloma cell growth and resistance to apoptosis. Additionally, this lysate is used to screen for chemical compounds that modulate these signaling pathways, providing insights into the cellular processes that drive multiple myeloma progression. By comparing U266 lysates with those from normal plasma cells, scientists can identify specific molecular alterations associated with malignancy. The use of U266 Whole Cell Lysate is essential for advancing our understanding of multiple myeloma biology and identifying potential molecular targets for further research.

U266 Whole Cell Lysate References:

1. Discovery of the catechol structural moiety as a Stat3 SH2 domain inhibitor by virtual screening.  |  Hao, W., et al. 2008. Bioorg Med Chem Lett. 18: 4988-92. PMID: 18768317