CCD-1064Sk Cell Lysate: sc-2263
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; skin fibroblast cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The CCD-1064Sk Cell Lysate is derived from the CCD-1064Sk cell line, which consists of human skin fibroblasts that have been cultivated under laboratory conditions. This lysate is a rich compilation of the various cellular components including proteins, nucleic acids, lipids, and metabolites, all extracted through comprehensive cell disruption techniques such as detergent lysis, sonication, or enzymatic digestion. The CCD-1064Sk Cell Lysate is particularly valued in biological research for its utility in studying the fundamental properties and behaviors of skin fibroblasts. Researchers leverage this lysate to explore cellular functions such as collagen synthesis, wound healing processes, and the response of skin cells to various environmental stimuli. It provides a crucial baseline for understanding the complex molecular interactions and signaling pathways that govern cell structure, growth, and differentiation in fibroblasts. Additionally, this lysate serves as an invaluable tool in proteomic studies, enabling the identification and quantification of proteins, and in molecular biology research for examining gene expression patterns. By using the CCD-1061Sk Cell Lysate, scientists gain insights into the basic cellular mechanisms that underpin skin physiology and cell biology, contributing significantly to the broader field of cellular and molecular research.
CCD-1064Sk Cell Lysate References:
- Retinoid-suppressed phosphorylation of RARalpha mediates the differentiation pathway of osteosarcoma cells. | Luo, P., et al. 2010. Oncogene. 29: 2772-83. PMID: 20190807
- 14-3-3 sigma associates with cell surface aminopeptidase N in the regulation of matrix metalloproteinase-1. | Ghaffari, A., et al. 2010. J Cell Sci. 123: 2996-3005. PMID: 20699358
- Antioxidant biomarkers from Vanda coerulea stems reduce irradiated HaCaT PGE-2 production as a result of COX-2 inhibition. | Simmler, C., et al. 2010. PLoS One. 5: e13713. PMID: 21060890
- Roles of type VI collagen and decorin in human mesenchymal stem cell biophysics during chondrogenic differentiation. | Twomey, JD., et al. 2014. Eur Cell Mater. 27: 237-50; discussion 249-50. PMID: 24668596
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCD-1064Sk Cell Lysate | sc-2263 | 500 µg/200 µl | $118.00 |