HL-60 + PMA Cell Lysate: sc-24705

HL-60 + PMA Cell Lysate is derived from the HL-60 cell line, a human promyelocytic leukemia cell line extensively utilized in the field of biomedical research, particularly in studies related to cell differentiation and signal transduction. The addition of Phorbol 12-myristate 13-acetate (PMA) to these cells triggers their differentiation into a more mature form, typically mimicking macrophage-like or granulocyte-like cells, depending on the specific experimental conditions. This differentiation process is of great interest because it allows researchers to study the cellular mechanisms and signaling pathways involved in the maturation of immune cells, which are crucial for understanding innate immune responses. The lysate itself, which is a complex mixture of proteins, nucleic acids, and other cellular components obtained by lysing these differentiated cells, serves as a rich source of biological molecules that can be analyzed to gain insights into protein expression patterns, post-translational modifications, and protein-protein interactions. Researchers often use this lysate in proteomics and biochemistry to investigate the cellular response to PMA, providing a deeper understanding of the molecular events that govern cell differentiation and function in a controlled laboratory setting. This makes HL-60 + PMA Cell Lysate an invaluable tool for elucidating the molecular landscape of cell development and behavior without direct implications for direct human application.