SK-N-SH + nocodazole Cell Lysate: sc-2289

SK-N-SH + nocodazole cell lysate is derived from a neuroblastoma cell line treated with nocodazole, a potent microtubule-depolymerizing agent. This combination provides a unique tool for investigating cellular responses to microtubule disruption, particularly in the context of neuronal cell types. Nocodazole's action in destabilizing microtubules leads to cell cycle arrest at the G2/M phase, enabling researchers to study checkpoints and mechanisms of cell cycle regulation. By using lysates from SK-N-SH cells treated with nocodazole, scientists can explore alterations in signaling pathways, such as those mediated by MAPK and PI3K/Akt, which are crucial for understanding cell survival and proliferation under stress conditions. Furthermore, this lysate facilitates the examination of molecular adaptations in neuroblastoma cells to cytoskeletal disruptions, including changes in protein expression and post-translational modifications like phosphorylation. Research applications extend to studying apoptosis induction mechanisms, as nocodazole treatment in this cell line provides insights into how neuroblastoma cells respond to cytotoxic stresses. This focus allows for a deeper understanding of basic cellular processes and molecular biology strictly within research parameters.