RAW 264.7 Cell Lysate for Immunoprecipaitation: sc-24798

RAW 264.7 (IP) cell lysate, derived from the RAW 264.7 murine macrophage cell line following immunoprecipitation (IP) procedures, is extensively used in proteomics and signal transduction research. This specialized lysate is crucial for identifying and analyzing protein-protein interactions and post-translational modifications that occur in macrophages during immune responses. Researchers utilize this lysate to delve into the detailed molecular mechanisms by which macrophages recognize and respond to pathogens. For instance, it helps in studying the assembly of signaling complexes at cellular receptors such as toll-like receptors (TLRs) after exposure to specific stimuli like LPS. The IP process enriches specific protein complexes, allowing for the investigation of interactions within the NF-κB pathway, a key mediator of inflammatory responses. Additionally, RAW 264.7 (IP) lysate is used to examine the activation of other signaling molecules such as MAPKs and the production of cytokines that drive the immune response. This research tool aids in uncovering the dynamics of macrophage activation and the regulatory networks that control macrophage functions, thereby advancing our understanding of immune regulation.

RAW 264.7 Cell Lysate for Immunoprecipaitation References:

1. Expression of prostaglandin (PG) D synthase lipocalin and hematopoietic type and PG D receptor during restart of spermatogenesis following downregulation using a slow release GnRH agonist implant in the dog.  |  Körber, H. and Goericke-Pesch, S. 2019. Cell Tissue Res. 378: 359-370. PMID: 31256286