U-87 MG Cell Lysate: sc-2411

U-87 MG Cell Lysate is derived from U-87 MG cells, a human glioblastoma cell line originally established from a malignant glioma patient. This lysate is widely used in cancer research to investigate the molecular mechanisms underlying glioblastoma multiforme (GBM), a highly aggressive brain tumor. U-87 MG cells exhibit alterations in several oncogenes and tumor suppressor genes, such as TP53, PTEN, and EGFR, making the lysate a valuable tool for studying signal transduction pathways involved in tumor growth, survival, and invasion. Researchers utilize U-87 MG Cell Lysate to analyze protein expression, phosphorylation, and interactions through techniques like western blotting, immunoprecipitation, and mass spectrometry. Key pathways studied include PI3K/AKT, MAPK/ERK, and mTOR, which are crucial for cell proliferation and resistance to apoptosis. Additionally, this lysate aids in understanding the molecular basis of glioma cell migration and invasion by examining the roles of integrins, matrix metalloproteinases, and cytoskeletal proteins. By comparing U-87 MG lysates with those from normal glial cells, scientists can identify specific molecular alterations associated with glioblastoma. This lysate is instrumental in advancing our knowledge of GBM biology, providing insights into potential molecular targets for further research in the field of cancer biology and signal transduction.

U-87 MG Cell Lysate References:

1. Expression and role of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate corpus luteum during the menstrual cycle.  |  Xu, J., et al. 2007. Endocrinology. 148: 5385-95. PMID: 17690168
2. Activation of Src protein tyrosine kinase plays an essential role in urocortin-mediated cardioprotection.  |  Yuan, Z., et al. 2010. Mol Cell Endocrinol. 325: 1-7. PMID: 20416357
3. Delivery of mirror image polypeptides into cells.  |  Rabideau, AE., et al. 2015. Chem Sci. 6: 648-653. PMID: 28706631
4. A Multi-targeted Natural Flavonoid Myricetin Suppresses Lamellipodia and Focal Adhesions Formation and Impedes Glioblastoma Cell Invasiveness and Abnormal Motility.  |  Zhao, HF., et al. 2018. CNS Neurol Disord Drug Targets. 17: 557-567. PMID: 29886836
5. Evaluation of immune and chemical precipitation methods for plasma exosome isolation.  |  Shtam, T., et al. 2020. PLoS One. 15: e0242732. PMID: 33232386
6. Cobalt oxide nanoparticle-synergized protein degradation and phototherapy for enhanced anticancer therapeutics.  |  Huang, X., et al. 2021. Acta Biomater. 121: 605-620. PMID: 33259958
7. Isolation, sequence and expression of a novel mouse brain cDNA, mIA-2, and its relatedness to members of the protein tyrosine phosphatase family.  |  Lu, J., et al. 1994. Biochem Biophys Res Commun. 204: 930-6. PMID: 7980563