HL-60 + DMSO Cell Lysate: sc-24703
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; acute promyelocytic leukemia cells induced with DMSO
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The HL-60 + DMSO Cell Lysate is derived from the HL-60 cell line, a human promyelocytic leukemia cell line originally established from a 36-year-old female patient. Treatment of these cells with dimethyl sulfoxide (DMSO) induces differentiation into granulocyte-like cells, making this lysate a critical tool in studying the differentiation process and myeloid cell biology. Researchers utilize the HL-60 + DMSO Cell Lysate to investigate the molecular mechanisms of differentiation, examining changes in gene expression, protein synthesis, and signal transduction pathways. This lysate is frequently used in proteomic studies, Western blotting, and mass spectrometry to identify proteins involved in the differentiation process and to understand their roles in cell signaling and function. It serves as a model system for evaluating the effects of various chemical compounds on myeloid differentiation, aiding in the identification of key regulatory molecules and pathways. Additionally, the lysate is instrumental in studying the interaction of differentiated cells with other cellular components and the extracellular matrix. The HL-60 + DMSO Cell Lysate provides a robust and reproducible platform for exploring the complex mechanisms underlying myeloid cell differentiation and for advancing research in hematopoiesis and leukemic cell biology.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HL-60 + DMSO Cell Lysate | sc-24703 | 500 µg/200 µl | $120.00 |