HOS Cell Lysate: sc-2275
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; osteosarcoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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HOS Cell Lysate originates from the HOS (Human Osteosarcoma) cell line, which is derived from human osteogenic sarcoma cells. This cell line has been a critical tool in the field of cancer biology and cellular research since its establishment. The lysate is prepared by lysing HOS cells, effectively breaking down the cell membranes to release the cellular contents, including proteins, enzymes, nucleic acids, and other intracellular molecules. Such lysates provide a comprehensive profile of the cell's biochemical state at the time of lysis, which is invaluable for in vitro experiments. Researchers utilize HOS Cell Lysates primarily to study the molecular and cellular mechanisms of bone formation and the pathological processes associated with osteosarcoma. The lysate serves as an essential resource for investigating cellular responses to various genetic and environmental stimuli, enabling detailed studies on signal transduction pathways and genetic regulation that are fundamental to understanding osteoblast biology. Moreover, its use in proteomics and genomics research helps in identifying biomarkers and understanding protein-protein interactions within osteosarcoma cells. This makes HOS Cell Lysates a vital component in non-therapeutic biological research, particularly in exploring fundamental cellular processes and mechanisms that guide cell behavior, proliferation, and differentiation in osteosarcoma-derived cells.
HOS Cell Lysate References:
- Manifestation of osteoblastic phenotypes in the sarcomatous component of epithelial carcinoma and sarcomatoid carcinoma. | Takashima, Y., et al. 2017. Tumour Biol. 39: 1010428317704365. PMID: 28651491
- Cell attachment to extracellular matrix substrates is inhibited upon downregulation of expression of calreticulin, an intracellular integrin alpha-subunit-binding protein. | Leung-Hagesteijn, CY., et al. 1994. J Cell Sci. 107 (Pt 3): 589-600. PMID: 8006073
- Molecular pathology of the cell cycle in human cancer cells. | Bártek, J., et al. 1993. Stem Cells. 11 Suppl 1: 51-8. PMID: 8318919
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HOS Cell Lysate | sc-2275 | 500 µg/200 µl | $118.00 |