HEL 92.1.7 Cell Lysate: sc-2270
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; erythroleukemia cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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SEE ALSO...
HEL 92.1.7 cell lysate is derived from the HEL 92.1.7 cell line, a human erythroleukemia cell line that is extensively used in hematological and cancer research. This cell line is particularly valuable for studying the molecular mechanisms of erythroid differentiation and leukemogenesis. Researchers utilize HEL 92.1.7 lysate to explore signaling pathways involved in erythropoiesis, such as the JAK/STAT pathway, which is crucial for mediating the response to erythropoietin, a key regulator of red blood cell production. The lysate is also instrumental in investigating the expression and function of oncogenes and tumor suppressor genes that play roles in leukemogenesis. For instance, the cell line is known to exhibit characteristics of both erythroid and megakaryocytic differentiation, making it a useful model for understanding the differentiation process and the dysregulation that leads to leukemia. Studies often focus on the impact of genetic mutations and chromosomal abnormalities present in the HEL 92.1.7 cells, such as alterations in the c-MYC and TP53 genes. Furthermore, HEL 92.1.7 lysate is used in proteomic analyses to identify protein expression profiles and post-translational modifications associated with cancer cell proliferation and survival.
HEL 92.1.7 Cell Lysate References:
- Measurement of serum p53 protein in patients with small cell lung cancer and results of its clinicopathological evaluation. | Segawa, Y., et al. 1997. Lung Cancer. 16: 229-38. PMID: 9152954
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HEL 92.1.7 Cell Lysate | sc-2270 | 500 µg/200 µl | $120.00 |