NRK Whole Cell Lysate: sc-364197

NRK whole cell lysate is derived from Normal Rat Kidney (NRK) cells, which are non-tumorigenic epithelial cells originally isolated from the kidneys of normal adult rats. This lysate is widely used in scientific research to study cellular signaling, cell growth, and differentiation mechanisms. NRK cells are valuable for investigating various aspects of epithelial cell biology and kidney function. Researchers employ NRK whole cell lysate to analyze key signaling pathways such as the MAPK/ERK, PI3K/Akt, and TGF-β pathways, which are crucial for regulating cell proliferation, survival, and differentiation. This lysate is instrumental in studying the expression and post-translational modifications of proteins associated with these pathways, as well as their interactions with other signaling molecules. Additionally, NRK whole cell lysate is used to investigate the effects of various chemical modulators on these signaling pathways, providing insights into the regulatory mechanisms of epithelial cell behavior and kidney function. Recent studies have utilized this lysate in proteomic and transcriptomic analyses to identify novel proteins and regulatory networks involved in epithelial cell biology. By offering detailed insights into the molecular and cellular mechanisms of epithelial cells and kidney function, NRK whole cell lysate continues to be a critical resource for advancing research in cell signaling and kidney biology.

NRK Whole Cell Lysate References:

1. Late endocytic compartments are major sites of annexin VI localization in NRK fibroblasts and polarized WIF-B hepatoma cells.  |  Pons, M., et al. 2000. Exp Cell Res. 257: 33-47. PMID: 10854052
2. Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation.  |  Solan, JL., et al. 2003. J Cell Sci. 116: 2203-11. PMID: 12697837
3. Identification of connexin-43 interacting proteins.  |  Singh, D. and Lampe, PD. 2003. Cell Commun Adhes. 10: 215-20. PMID: 14681019
4. Connexin phosphorylation as a regulatory event linked to gap junction channel assembly.  |  Solan, JL. and Lampe, PD. 2005. Biochim Biophys Acta. 1711: 154-63. PMID: 15955300
5. Connexin 43 interacts with zona occludens-1 and -2 proteins in a cell cycle stage-specific manner.  |  Singh, D., et al. 2005. J Biol Chem. 280: 30416-21. PMID: 15980428
6. Simian sarcoma virus transformation of normal rat kidney fibroblasts is associated with markedly increased basic fibroblast growth factor expression.  |  Milner, PG. 1991. Biochem Biophys Res Commun. 180: 423-30. PMID: 1656977
7. Characterization of Na+/H+ exchanger NHE8 in cultured renal epithelial cells.  |  Zhang, J., et al. 2007. Am J Physiol Renal Physiol. 293: F761-6. PMID: 17581925
8. The C-terminus of connexin43 adopts different conformations in the Golgi and gap junction as detected with structure-specific antibodies.  |  Sosinsky, GE., et al. 2007. Biochem J. 408: 375-85. PMID: 17714073
9. Role of the prostanoid FP receptor in action potential generation and phenotypic transformation of NRK fibroblasts.  |  Almirza, WH., et al. 2008. Cell Signal. 20: 2022-9. PMID: 18703136
10. Effects of cadmium on the sub-cellular localization of β-catenin and β-catenin-regulated gene expression in NRK-52E cells.  |  Edwards, JR., et al. 2013. Biometals. 26: 33-42. PMID: 23080431
11. The first direct activity assay for the mitochondrial protease OMA1.  |  Tobacyk, J., et al. 2019. Mitochondrion. 46: 1-5. PMID: 30926535
12. NHE8 attenuates Ca2+ influx into NRK cells and the proximal tubule epithelium.  |  Wiebe, SA., et al. 2019. Am J Physiol Renal Physiol. 317: F240-F253. PMID: 31042050
13. Retinoic Acid Alleviates Cisplatin-Induced Acute Kidney Injury Through Activation of Autophagy.  |  Wu, J., et al. 2020. Front Pharmacol. 11: 987. PMID: 32719599
14. Cell locomotion and focal adhesions are regulated by substrate flexibility.  |  Pelham, RJ. and Wang, Yl. 1997. Proc Natl Acad Sci U S A. 94: 13661-5. PMID: 9391082