GM637 Whole Cell Lysate: sc-364361
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; SV40-transformed fibroblast cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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GM637 whole cell lysate is derived from the GM637 cell line, which originates from normal human fibroblasts. This lysate is primarily utilized in genetic and cellular research to investigate the fundamental processes of cell cycle regulation, DNA repair mechanisms, and responses to genotoxic stress. The GM637 cell line, being a non-cancerous line, provides a baseline for understanding normal cellular physiology and contrasts with cancerous cell lines in comparative studies. Researchers employ GM637 lysate to study the efficiency and pathways involved in DNA repair, particularly focusing on homologous recombination and non-homologous end joining. It is also used to investigate the cellular response to various forms of DNA damage, such as those induced by UV radiation and chemical agents, allowing for a detailed analysis of the activation and regulation of key proteins involved in the DNA damage response, including p53, ATM, and ATR. Additionally, this lysate aids in exploring the mechanisms of senescence and the impact of oxidative stress on cellular aging. By utilizing GM637 whole cell lysate, scientists gain insights into the molecular underpinnings of cellular maintenance and genome integrity, advancing our knowledge of cellular biology and genetic stability.
GM637 Whole Cell Lysate References:
- Distinct functional domains of nibrin mediate Mre11 binding, focus formation, and nuclear localization. | Desai-Mehta, A., et al. 2001. Mol Cell Biol. 21: 2184-91. PMID: 11238951
- Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination. | Watanabe, K., et al. 2004. EMBO J. 23: 3886-96. PMID: 15359278
- RECQL4-deficient cells are hypersensitive to oxidative stress/damage: Insights for osteosarcoma prevalence and heterogeneity in Rothmund-Thomson syndrome. | Werner, SR., et al. 2006. Biochem Biophys Res Commun. 345: 403-9. PMID: 16678792
- Expression of DNA translesion synthesis polymerase η in head and neck squamous cell cancer predicts resistance to gemcitabine and cisplatin-based chemotherapy. | Zhou, W., et al. 2013. PLoS One. 8: e83978. PMID: 24376779
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GM637 Whole Cell Lysate | sc-364361 | 500 µg/200 µl | $118.00 |