PC-12 Cell Lysate: sc-2250

PC-12 cell lysate, derived from the rat pheochromocytoma PC-12 cell line, is widely used in scientific research to study neuronal differentiation and signal transduction mechanisms. PC-12 cells are known for their ability to respond to nerve growth factor (NGF), which induces differentiation into neuron-like cells, making the lysate a valuable tool for investigating neurobiological processes. Researchers utilize PC-12 cell lysate to examine the phosphorylation of various proteins involved in NGF signaling pathways, such as the ERK/MAPK cascade, which plays a crucial role in cell differentiation, survival, and proliferation. Additionally, this lysate is employed to study the regulation of transcription factors like CREB and AP-1, which are essential for neuronal growth and synaptic plasticity. Recent studies have leveraged PC-12 cell lysate to explore the effects of chemical inhibitors and activators on protein kinase activities, providing insights into the modulation of signaling pathways. This lysate is also instrumental in proteomic analyses to identify novel proteins and post-translational modifications involved in neuronal function. By enabling detailed mechanistic studies, PC-12 cell lysate continues to be a vital resource for advancing our understanding of neurobiology and cellular signaling in a controlled experimental environment.

PC-12 Cell Lysate References:

1. Screening of ion channel receptor agonists using capillary electrophoresis-patch clamp detection with resensitized detector cells.  |  Farre, C., et al. 2001. Anal Chem. 73: 1228-33. PMID: 11305656
2. Akt-mediated valosin-containing protein 97 phosphorylation regulates its association with ubiquitinated proteins.  |  Klein, JB., et al. 2005. J Biol Chem. 280: 31870-81. PMID: 16027165
3. Microtubule-associated protein 1B-light chain 1 enhances activation of Rap1 by exchange protein activated by cyclic AMP but not intracellular targeting.  |  Borland, G., et al. 2006. Mol Pharmacol. 69: 374-84. PMID: 16244178
4. Relationship between drug release of DE-310, macromolecular prodrug of DX-8951f, and cathepsins activity in several tumors.  |  Shiose, Y., et al. 2007. Biol Pharm Bull. 30: 2365-70. PMID: 18057727
5. Collapsin response mediator protein-2 is a calmodulin-binding protein.  |  Zhang, Z., et al. 2009. Cell Mol Life Sci. 66: 526-36. PMID: 19151921
6. Actin involvement in exocytosis from PC12 cells: studies on the influence of botulinum C2 toxin on stimulated noradrenaline release.  |  Matter, K., et al. 1989. J Neurochem. 52: 370-6. PMID: 2492057
7. Identification of novel autophagic Radix Polygalae fraction by cell membrane chromatography and UHPLC-(Q)TOF-MS for degradation of neurodegenerative disease proteins.  |  Wu, AG., et al. 2015. Sci Rep. 5: 17199. PMID: 26598009
8. Modulation activity of heat-treated and untreated lactoferrin on the TLR-4 pathway in anoxia cell model and cerebral ischemia reperfusion mouse model.  |  Yang, HG., et al. 2020. J Dairy Sci. 103: 1151-1163. PMID: 31837800
9. Identification of cDNA clones coding for rat tyrosine hydroxylase antigen.  |  Lamouroux, A., et al. 1982. Proc Natl Acad Sci U S A. 79: 3881-5. PMID: 6179090
10. Generation of angiotensins in cultured pheochromocytoma cells.  |  Okamura, T., et al. 1984. Neurosci Lett. 46: 151-6. PMID: 6330620
11. A bryostatin-sensitive protein kinase C required for nerve growth factor activity.  |  Singh, KR., et al. 1994. Biochemistry. 33: 542-51. PMID: 8286384
12. Amino-terminal region of the beta-amyloid precursor protein activates mitogen-activated protein kinase.  |  Greenberg, SM., et al. 1995. Neurosci Lett. 198: 52-6. PMID: 8570096
13. Alpha-subunit of farnesyltransferase is phosphorylated in vivo: effect of protein phosphatase-1 on enzymatic activity.  |  Kumar, A., et al. 1996. Biochem Biophys Res Commun. 222: 445-52. PMID: 8670225