SK-N-SH Cell Lysate: sc-2410

SK-N-SH cell lysate originates from a human neuroblastoma cell line, widely utilized in neurobiology and cancer research due to its distinct expression of neuronal and adrenergic markers. This lysate serves as a rich source of cellular components, enabling researchers to study protein interactions, post-translational modifications, and signaling pathways relevant to neuroblastoma and other neurologically related conditions. In studies, SK-N-SH cell lysate has been instrumental in the investigation of intracellular pathways, such as those involving MYCN, a gene significantly amplified in neuroblastoma that influences cell cycle progression and apoptosis. Researchers also use this lysate to analyze the effect of various chemical compounds on cellular processes like differentiation and proliferation. Furthermore, it aids in the screening for molecules that can modulate the expression of specific proteins associated with neuroblastoma pathophysiology. The application of SK-N-SH cell lysate in these areas contributes to a deeper understanding of cellular mechanisms, focusing purely on the biochemical and molecular dynamics within research environments.

SK-N-SH Cell Lysate References:

1. H9724, a monoclonal antibody to Borrelia burgdorferi′s flagellin, binds to heat shock protein 60 (HSP60) within live neuroblastoma cells: a potential role for HSP60 in peptide hormone signaling and in an autoimmune pathogenesis of the neuropathy of Lyme disease.  |  Sigal, LH., et al. 2001. Cell Mol Neurobiol. 21: 477-95. PMID: 11860186
2. Neuronal protein NP25 interacts with F-actin.  |  Mori, K., et al. 2004. Neurosci Res. 48: 439-46. PMID: 15041197
3. Determination of high-affinity choline uptake (HACU) and choline acetyltransferase (ChAT) activity in the same population of cultured cells.  |  Ray, B., et al. 2009. Brain Res. 1297: 160-8. PMID: 19660442
4. Apolipoprotein-E forms dimers in human frontal cortex and hippocampus.  |  Elliott, DA., et al. 2010. BMC Neurosci. 11: 23. PMID: 20170526
5. Antibody fragments directed against different portions of the human neural cell adhesion molecule L1 act as inhibitors or activators of L1 function.  |  Wang, Y., et al. 2012. PLoS One. 7: e52404. PMID: 23272240
6. AhR-mediated effects of dioxin on neuronal acetylcholinesterase expression in vitro.  |  Xie, HQ., et al. 2013. Environ Health Perspect. 121: 613-8. PMID: 23426015
7. Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex.  |  Yang, W., et al. 2013. Neurochem Int. 63: 180-94. PMID: 23796501
8. The uncoupling of synaptic protein homer 1c from target proteins activates store-operated calcium entry in a neurotransmitter-like manner in human neuroblastoma cells.  |  Ryazantseva, MA., et al. 2013. Dokl Biol Sci. 450: 181-4. PMID: 23821062
9. Very late activation antigens (VLA) are human leukocyte-neuronal crossreactive cell surface antigens.  |  Pischel, KD., et al. 1986. J Exp Med. 164: 393-406. PMID: 2425033
10. Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography.  |  Li, Y., et al. 2018. Talanta. 178: 811-817. PMID: 29136899
11. LINC00943 knockdown exerts neuroprotective effects in Parkinson′s disease through regulates CXCL12 expression by sponging miR-7-5p.  |  Lian, H., et al. 2021. Genes Genomics. 43: 797-805. PMID: 33886117
12. Biallelic inheritance in a single Pakistani family with intellectual disability implicates new candidate gene RDH14.  |  Pastore, SF., et al. 2021. Sci Rep. 11: 23113. PMID: 34848785
13. A virus with a mutation in the ICP4-binding site in the L/ST promoter of herpes simplex virus type 1, but not a virus with a mutation in open reading frame P, exhibits cell-type-specific expression of gamma(1)34.5 transcripts and latency-associated transcripts.  |  Lee, LY. and Schaffer, PA. 1998. J Virol. 72: 4250-64. PMID: 9557715