mouse LacZ Whole Cell Lysate: sc-364371

Mouse LacZ whole cell lysate is derived from mouse fibroblast cells transformed with the SV-40 virus. This lysate is extensively used in research to study gene expression, protein localization, and cellular signaling pathways. The LacZ gene, when expressed, produces β-galactosidase, an enzyme that cleaves X-gal into a blue product, allowing for easy visualization and quantification of gene expression. Researchers utilize mouse LacZ whole cell lysate to analyze the activity of β-galactosidase as a reporter for promoter activity, enhancer function, and signal transduction pathways. This lysate facilitates the study of protein expression, post-translational modifications, and the interactions between signaling molecules involved in these pathways. Additionally, it is used to examine the effects of various chemical modulators on gene expression and protein activity, providing insights into the regulatory mechanisms of cellular processes. Recent studies have leveraged mouse LacZ whole cell lysate for proteomic and genomic analyses to identify novel proteins and signaling networks involved in gene regulation and cellular functions. By offering detailed insights into the molecular mechanisms of gene expression, mouse LacZ whole cell lysate remains a valuable resource for advancing research in cell biology and molecular genetics.