A-375 Cell Lysate: sc-3811
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; malignant melanoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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A-375 cell lysate is derived from the A-375 cell line, which is a human melanoma cell line originally established from a skin lesion of a 54-year-old female patient. These cells are widely utilized in the field of cancer research, particularly in studies focusing on melanoma, a form of skin cancer known for its high metastatic potential and resistance to traditional treatments. The lysate itself is a preparation that includes the whole cell contents obtained after lysing, or breaking down, the A-375 cells. This process releases a variety of cellular components, including proteins, DNA, RNA, and other biomolecules, which are crucial for various biochemical and molecular biology experiments. Researchers use A-375 cell lysate extensively in proteomics to identify and quantify protein expression levels, study protein interactions, and observe the effects of gene manipulation. In addition, this lysate serves as a valuable control in Western blotting, immunoprecipitation, and other assay techniques that require a comprehensive understanding of the cellular components and their functions. By providing a snapshot of the cellular environment, A-375 cell lysate aids researchers in elucidating the molecular pathways and mechanisms underlying melanoma progression and in exploring new avenues for basic scientific inquiry.
A-375 Cell Lysate References:
- The p34cdc2-related cyclin-dependent kinase 11 interacts with the p47 subunit of eukaryotic initiation factor 3 during apoptosis. | Shi, J., et al. 2003. J Biol Chem. 278: 5062-71. PMID: 12446680
- RKTG sequesters B-Raf to the Golgi apparatus and inhibits the proliferation and tumorigenicity of human malignant melanoma cells. | Fan, F., et al. 2008. Carcinogenesis. 29: 1157-63. PMID: 18515281
- Generation and characterization of a panel of monoclonal antibodies against distinct epitopes of human CD146. | Zhang, Y., et al. 2008. Hybridoma (Larchmt). 27: 345-52. PMID: 18847347
- A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1. | Narayan, V., et al. 2011. J Biol Chem. 286: 14291-303. PMID: 21245151
- In situ kinase profiling reveals functionally relevant properties of native kinases. | Patricelli, MP., et al. 2011. Chem Biol. 18: 699-710. PMID: 21700206
- Recognition of CD146 as an ERM-binding protein offers novel mechanisms for melanoma cell migration. | Luo, Y., et al. 2012. Oncogene. 31: 306-21. PMID: 21725352
- CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry. | Lai, CY., et al. 2012. Cancer Res. 72: 5111-8. PMID: 22865455
- Exploiting the MDM2-CK1α protein-protein interface to develop novel biologics that induce UBL-kinase-modification and inhibit cell growth. | Huart, AS., et al. 2012. PLoS One. 7: e43391. PMID: 22916255
- Discovery and synthesis of the first selective BAG domain modulator of BAG3 as an attractive candidate for the development of a new class of chemotherapeutics. | Terracciano, S., et al. 2018. Chem Commun (Camb). 54: 7613-7616. PMID: 29926854
- Identification and Functional Evaluation of miR-4633-5p as a Biomarker and Tumor Suppressor in Metastatic Melanoma. | Zou, B., et al. 2018. Cell Physiol Biochem. 49: 1364-1379. PMID: 30205394
- LncRNA GAS5 regulates redox balance and dysregulates the cell cycle and apoptosis in malignant melanoma cells. | Chen, L., et al. 2019. J Cancer Res Clin Oncol. 145: 637-652. PMID: 30569211
- Selective Anti-melanoma Effect of Phosphothioated Aptamer Encapsulated by Neutral Cytidinyl/Cationic Lipids. | Wu, J., et al. 2021. Front Cell Dev Biol. 9: 660233. PMID: 34262898
- Extracellular 20S proteasome secreted via microvesicles can degrade poorly folded proteins and inhibit Galectin-3 agglutination activity. | Bonhoure, A., et al. 2022. Traffic. 23: 287-304. PMID: 35466519
- Macromolecular Crowding as an Intracellular Stimulus for Responsive Nanomaterials. | Estabrook, DA., et al. 2022. J Am Chem Soc. 144: 16792-16798. PMID: 36084194
- Ginkgo biloba Golden Leaf Extract (GGLE) Inhibits Melanoma Cell Invasion and Angiogenesis Through Inhibition of Angiogenin. | Chen, P., et al. 2023. Integr Cancer Ther. 22: 15347354221134513. PMID: 36859800
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A-375 Cell Lysate | sc-3811 | 500 µg/200 µl | $118.00 |