MCF7 + insulin Cell Lysate: sc-24733

MCF7 + insulin cell lysate is derived from the MCF7 breast cancer cell line treated with insulin to study the effects of insulin on ER-positive breast cancer cells, focusing on insulin's role in modulating cellular metabolism and growth signaling pathways. This lysate provides a crucial tool for examining how insulin interacts with the estrogen receptor (ER) pathway and other signaling networks, such as the PI3K/Akt and MAPK pathways, which are known to influence cell proliferation and survival. Researchers use this specific lysate to investigate the cross-talk between insulin and estrogen signaling, which can lead to enhanced cell cycle progression and anti-apoptotic activity in breast cancer cells. This is particularly relevant in understanding how hyperinsulinemia might affect breast cancer progression in diabetic conditions. The MCF7 + insulin lysate allows for the detailed study of insulin's impact on gene expression changes, protein phosphorylation, and the activation state of various signaling molecules involved in cancer cell metabolism and growth. By focusing on these biochemical pathways in a strictly research-oriented context, the lysate helps explain the complex interactions that drive cancer cell behavior in response to metabolic hormones. This research provides deep insights into the molecular mechanisms of hormone interaction in cancer biology.