HUV-EC-C + VEGF Cell Lysate: sc-24709
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; vascular endothelial cells induced with VEGF
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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HUV-EC-C + VEGF Cell Lysate is derived from the HUV-EC-C cell line, which is a line of human umbilical vein endothelial cells (HUVECs), cultured in the presence of Vascular Endothelial Growth Factor (VEGF). This cell line is crucial for studying endothelial function and vascular biology since HUVECs are representative of endothelial cells lining the veins and are central to vascular health and pathology. VEGF, a significant regulator of vascular development and angiogenesis, is added to stimulate pathways relevant to these processes, making the lysate particularly rich in signaling molecules involved in vascular growth and repair. The lysate itself is produced by lysing these cells, which involves breaking down the cell membranes to release cellular contents including proteins, DNA, and other cellular molecules which reflect the cell's state under the influence of VEGF. This makes it an invaluable tool for researchers studying the mechanisms of angiogenesis, endothelial cell function, and the cellular response to growth factors. In research, this lysate has been used to understand how endothelial cells contribute to new blood vessel formation, the cellular responses to hypoxic conditions, and the regulation of vascular permeability, providing insights into the basic mechanisms of vascular biology and endothelial dynamics.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HUV-EC-C + VEGF Cell Lysate | sc-24709 | 500 µg/200 µl | $118.00 |