L929 Cell Lysate: sc-24729

L929 Cell Lysate is derived from the L929 cell line, a fibroblast-like cell line originally established from subcutaneous connective tissues of a C3H/An mouse. This cell line has been historically significant in the field of cellular biology for several decades, especially in studies related to cellular morphology, growth characteristics, and cytokine production. The L929 Cell Lysate contains a wide array of cellular components such as proteins, nucleic acids, lipids, and other metabolites, obtained through the lysis of L929 cells. This rich biochemical mixture is utilized extensively in research to study various aspects of cell biology, particularly in understanding the pathways of necrosis and apoptosis, cellular response to external stimuli, and cytokine interactions. Researchers employ L929 Cell Lysate in assays to explore gene expression, signal transduction pathways, and protein-protein interactions, providing insights into the underlying mechanisms of cell communication and response. Furthermore, this lysate serves as an essential control in numerous experimental setups, such as Western blotting and enzyme-linked immunosorbent assays (ELISA), helping to benchmark and validate experimental results in a variety of cellular processes studies.

L929 Cell Lysate References:

1. Dual role of caspase-11 in mediating activation of caspase-1 and caspase-3 under pathological conditions.  |  Kang, SJ., et al. 2000. J Cell Biol. 149: 613-22. PMID: 10791975
2. Expression of chemokine genes in human dermal microvascular endothelial cell lines infected with Orientia tsutsugamushi.  |  Cho, NH., et al. 2001. Infect Immun. 69: 1265-72. PMID: 11179287
3. Direct involvement of CREB-binding protein/p300 in sequence-specific DNA binding of virus-activated interferon regulatory factor-3 holocomplex.  |  Suhara, W., et al. 2002. J Biol Chem. 277: 22304-13. PMID: 11940575
4. Cultured mesangial cells from autoimmune MRL-lpr mice have decreased secreted and surface M-CSF.  |  Brennan, DC., et al. 1992. Kidney Int. 42: 279-84. PMID: 1405313
5. Reovirus delays diabetes onset but does not prevent insulitis in nonobese diabetic mice.  |  Wetzel, JD., et al. 2006. J Virol. 80: 3078-82. PMID: 16501117
6. Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin beta.  |  Echeverría, PC., et al. 2009. Mol Cell Biol. 29: 4788-97. PMID: 19581287
7. Legionella pneumophila requires polyamines for optimal intracellular growth.  |  Nasrallah, GK., et al. 2011. J Bacteriol. 193: 4346-60. PMID: 21742865
8. SWATH enables precise label-free quantification on proteome scale.  |  Huang, Q., et al. 2015. Proteomics. 15: 1215-23. PMID: 25560523
9. Exosomes from Dendritic Cells Loaded with Chaperone-Rich Cell Lysates Elicit a Potent T Cell Immune Response Against Intracranial Glioma in Mice.  |  Bu, N., et al. 2015. J Mol Neurosci. 56: 631-43. PMID: 25680514
10. Temperature-dependent oligomerization of hsp85 in vitro.  |  Lanks, KW. 1989. J Cell Physiol. 140: 601-7. PMID: 2777893
11. Systematic Assessment of the Effect of Internal Library in Targeted Analysis of SWATH-MS.  |  Zhong, CQ., et al. 2020. J Proteome Res. 19: 477-492. PMID: 31664839
12. Quantitation of S-adenosylhomocysteine hydrolase in mouse L929 cells using the inhibitor neplanocin A.  |  Bartel, RL. and Borchardt, RT. 1985. Anal Biochem. 149: 191-6. PMID: 4073475
13. Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells.  |  Lanks, KW. and Kasambalides, EJ. 1983. J Cell Biol. 96: 577-81. PMID: 6300137
14. A major cytoplasmic glucose-regulated protein is associated with the Rous sarcoma virus pp60src protein.  |  Lanks, KW., et al. 1982. J Biol Chem. 257: 8604-7. PMID: 6807976
15. Spontaneous cell hybridization of somatic cells present in sperm suspensions.  |  Phillips, SG., et al. 1976. Exp Cell Res. 98: 429-43. PMID: 943304