A-431 + EGF Cell Lysate for Immunoprecipaitation: sc-24782

The A-431 + EGF (IP) cell lysate is obtained from the A-431 cell line, which originates from an epidermoid carcinoma in an 85-year-old female. This particular lysate is enhanced by the addition of Epidermal Growth Factor (EGF), a key regulator in cell growth and differentiation, and is prepared specifically for immunoprecipitation (IP) experiments. The A-431 cells, known for their high expression of the Epidermal Growth Factor Receptor (EGFR), provide a reactive system for studying EGF-EGFR interactions and the subsequent activation of signaling pathways. The inclusion of EGF in this lysate promotes the activation of these receptors, leading to a cascade of phosphorylation events that are critical for understanding cellular responses to growth signals. This lysate is particularly valuable in research focused on signal transduction pathways, allowing scientists to pull down and study specific proteins that are involved in the signaling pathway initiated by EGF. By using this lysate in immunoprecipitation protocols, researchers can isolate and analyze the behavior of proteins and complexes involved in these pathways, providing insights into cellular mechanisms of action, signal transduction, and regulation. This makes the A-431 + EGF (IP) lysate an essential tool in basic biological research and in studies exploring fundamental aspects of cellular communication and regulation.

A-431 + EGF Cell Lysate for Immunoprecipaitation References:

1. Selective isolation of N-terminal peptides from proteins and their de novo sequencing by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry without regard to unblocking or blocking of N-terminal amino acids.  |  Yamaguchi, M., et al. 2008. Rapid Commun Mass Spectrom. 22: 3313-9. PMID: 18821723