A549 Cell Lysate: sc-2413
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; lung carcinoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The A549 Cell Lysate originates from a human alveolar basal epithelial cell line, commonly utilized in research focused on pulmonary biology. This lysate is derived from cells first isolated in 1972 from the lung tissue of a 58-year-old Caucasian male. The utility of the A549 lysate in research stems from its ability to model the cellular environment of lung epithelial cells, offering crucial insights into cellular processes such as gas exchange, fluid balance, and response to pathogens. Researchers employ this lysate to study gene expression and protein networks involved in respiratory physiology and responses to environmental stresses. In molecular biology, the A549 lysate provides a valuable reference material for protein identification and quantification, enabling scientists to perform comparative analyses and validate experimental findings related to cellular responses under various conditions. Its use in proteomics, particularly through techniques like Western blotting and mass spectrometry, helps in elucidating protein-protein interactions and signaling pathways. Additionally, the lysate serves as a critical component in studies exploring the effects of pollutants, allergens, and other airborne substances on lung cells, contributing significantly to our understanding of respiratory health and disease mechanisms at the molecular level.
A549 Cell Lysate References:
- Does pH 6 beta-galactosidase activity indicate cell senescence? | Krishna, DR., et al. 1999. Mech Ageing Dev. 109: 113-23. PMID: 10515661
- Inactivation of a Pseudomonas aeruginosa quorum-sensing signal by human airway epithelia. | Chun, CK., et al. 2004. Proc Natl Acad Sci U S A. 101: 3587-90. PMID: 14970327
- Cytoplasmic dynein mediates adenovirus binding to microtubules. | Kelkar, SA., et al. 2004. J Virol. 78: 10122-32. PMID: 15331745
- Influenza A virus non-structural protein 1 (NS1) interacts with cellular multifunctional protein nucleolin during infection. | Murayama, R., et al. 2007. Biochem Biophys Res Commun. 362: 880-5. PMID: 17767916
- Autoantibody to alanyl-tRNA synthetase in patients with idiopathic pulmonary fibrosis. | Takahashi, T., et al. 2007. Respirology. 12: 642-53. PMID: 17875050
- Measurement of intracellular accumulation of anthracyclines in cancerous cells by direct injection of cell lysate in MEKC/LIF detection. | Mbuna, J., et al. 2010. Electrophoresis. 31: 1396-404. PMID: 20217860
- Nanoparticles attenuate P-glycoprotein/MDR1 function in A549 human alveolar epithelial cells. | Salomon, JJ. and Ehrhardt, C. 2011. Eur J Pharm Biopharm. 77: 392-7. PMID: 21093586
- PX-12 inhibits the growth of A549 lung cancer cells via G2/M phase arrest and ROS-dependent apoptosis. | You, BR., et al. 2014. Int J Oncol. 44: 301-8. PMID: 24172913
- Visual detection of microRNA with lateral flow nucleic acid biosensor. | Gao, X., et al. 2014. Biosens Bioelectron. 54: 578-84. PMID: 24333569
- An enzyme-amplified lateral flow strip biosensor for visual detection of microRNA-224. | Gao, X., et al. 2016. Talanta. 146: 648-54. PMID: 26695313
- Analysis of A549 cell proteome alteration in response to recombinant influenza A virus nucleoprotein and its interaction with cellular proteins, a preliminary study. | Kumar, D., et al. 2017. Acta Virol. 61: 56-65. PMID: 28105855
- Design, synthesis, and characterization of α, β-unsaturated carboxylic acid, and its urea based derivatives that explores novel epigenetic modulators in human non-small cell lung cancer A549 cell line. | Chidambaram, A., et al. 2018. J Cell Physiol. 233: 5293-5309. PMID: 29215703
- Bioinspired DNA-Inorganic Hybrid Nanoflowers Combined with a Personal Glucose Meter for Onsite Detection of miRNA. | Wu, T., et al. 2018. ACS Appl Mater Interfaces. 10: 42050-42057. PMID: 30457317
- A microfluidic paper-based laser-induced fluorescence sensor based on duplex-specific nuclease amplification for selective and sensitive detection of miRNAs in cancer cells. | Cai, X., et al. 2020. Talanta. 216: 120996. PMID: 32456922
- Anti-epithelial (anti-A549) antibodies: their nature, specificity and relevance to transplantation. | Abdul-Karim, AB., et al. 1995. Transpl Immunol. 3: 192-202. PMID: 8581407
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A549 Cell Lysate | sc-2413 | 500 µg/200 µl | $118.00 |