WI-38 Whole Cell Lysate: sc-364260

WI-38 is a diploid human cell line composed of fibroblasts derived from lung tissue of a 3-month-gestation female fetus. This lysate is widely used in research to study cellular aging, virus-host interactions, and the molecular mechanisms of fibroblast biology. WI-38 cells are known for their normal diploid karyotype and limited lifespan in culture, making the lysate a valuable resource for investigating the processes of cellular senescence and the response to various stressors. Researchers utilize WI-38 Whole Cell Lysate to analyze the expression and modification of key proteins involved in cell cycle regulation, DNA repair, and apoptosis using techniques such as western blotting, immunoprecipitation, and mass spectrometry. The lysate helps in studying the activation states and interactions of signaling pathways such as p53/p21 and the retinoblastoma (Rb) pathway, which are critical in maintaining genomic integrity and cellular homeostasis. Additionally, WI-38 Whole Cell Lysate is employed in research on virus replication and pathogenesis, as these cells are permissive to several human viruses. By providing a comprehensive profile of cellular components, this lysate aids in elucidating the complex molecular networks that govern cell proliferation, differentiation, and response to external stimuli, thereby advancing our understanding of cellular biology and aging.