CCD-1064Sk + PDGF Cell Lysate: sc-2264
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; skin fibroblast cells induced with PDGF
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The CCD-1064Sk + PDGF Cell Lysate is derived from the CCD-1064Sk cell line, which is a line of human skin fibroblasts, specifically stimulated with Platelet-Derived Growth Factor (PDGF). This lysate contains a complex array of molecular components including proteins, nucleic acids, and other cellular constituents extracted via methods such as sonication, detergent-based lysis, or enzymatic cleavage. The addition of PDGF to these cells prior to lysis is crucial as PDGF is a known potent mitogen for fibroblasts, playing a significant role in cellular processes such as proliferation and migration. This lysate is particularly valuable in research focused on studying the signaling pathways and cellular behaviors induced by growth factors. Researchers utilize this lysate to investigate the direct biochemical and cellular effects of PDGF on fibroblasts, including changes in protein phosphorylation, gene expression, and the cellular machinery involved in migration and proliferation. By providing insights into the basic cellular responses to growth factors, CCD-1064Sk + PDGF Cell Lysate facilitates a deeper understanding of cell communication and regulation, particularly in the context of cellular growth and repair mechanisms.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCD-1064Sk + PDGF Cell Lysate | sc-2264 | 500 µg/200 µl | $118.00 |