A-431 Whole Cell Lysate: sc-2201
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; epidermoid carcinoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The A-431 Whole Cell Lysate is produced from the A-431 cell line, which originates from an epidermoid carcinoma of the skin from an 85-year-old female. This lysate is a comprehensive preparation containing all the cellular components extracted from A-431 cells, thus providing a rich source of cellular material for research. It includes proteins, nucleic acids, lipids, and other small molecules, making it extremely useful for a wide range of biochemical and molecular biology investigations. Researchers utilize this lysate to study protein expression profiles, investigate enzyme activities, and analyze cellular structures and functions under various experimental conditions. The A-431 cell line itself is particularly noted for its high expression of the epidermal growth factor receptor (EGFR), making the lysate ideal for studying EGFR signaling pathways without the complexity of isolating specific proteins or complexes. This allows scientists to explore cellular responses in a system that closely mimics the natural state of the cells. The A-431 Whole Cell Lysate has been instrumental in advancing our understanding of cellular mechanisms and protein interactions, facilitating explorations into how cells communicate, respond to environmental stimuli, and regulate various metabolic and signal transduction pathways. This lysate serves as a crucial tool in basic research, particularly in fields such as cell biology and proteomics.
A-431 Whole Cell Lysate References:
- Affinity enrichment of plasma membrane for proteomics analysis. | Zhang, W., et al. 2003. Electrophoresis. 24: 2855-63. PMID: 12929181
- Assembly of alpha-hemolysin on A431 cells leads to clustering of Caveolin-1. | Vijayvargia, R., et al. 2004. Biochem Biophys Res Commun. 324: 1124-9. PMID: 15485671
- Stress-induced phosphorylation of caveolin-1 and p38, and down-regulation of EGFr and ERK by the dietary lectin jacalin in two human carcinoma cell lines. | Sahasrabuddhe, AA., et al. 2006. Cell Stress Chaperones. 11: 135-47. PMID: 16817319
- A clean, more efficient method for in-solution digestion of protein mixtures without detergent or urea. | Kim, SC., et al. 2006. J Proteome Res. 5: 3446-52. PMID: 17137347
- Three-dimensional culture using a radial flow bioreactor induces matrix metalloprotease 7-mediated EMT-like process in tumor cells via TGFbeta1/Smad pathway. | Shibata, S., et al. 2009. Int J Oncol. 34: 1433-48. PMID: 19360357
- Modulation of PP2A activity by Jacalin: is it through caveolae and ER chaperones? | Ahmed, N., et al. 2010. Glycoconj J. 27: 723-34. PMID: 19823931
- Low ascorbate levels are associated with increased hypoxia-inducible factor-1 activity and an aggressive tumor phenotype in endometrial cancer. | Kuiper, C., et al. 2010. Cancer Res. 70: 5749-58. PMID: 20570889
- Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines. | Moll, R., et al. 1988. Am J Pathol. 132: 123-44. PMID: 2456018
- Indomethacin inhibits activation of endothelial nitric oxide synthase in the rat kidney: possible role of this effect in the pathogenesis of indomethacin-induced renal damage. | Nagappan, AS., et al. 2014. Chem Biol Interact. 221: 77-87. PMID: 25110317
- Separation of novel phosphoproteins of Porphyromonas gingivalis using phosphate-affinity chromatography. | Izumigawa, M., et al. 2016. Microbiol Immunol. 60: 702-707. PMID: 27663267
- Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays. | Yang, Y. and Zeng, Y. 2018. Lab Chip. 18: 3830-3839. PMID: 30394473
- Selective and reversible modification of kinase cysteines with chlorofluoroacetamides. | Shindo, N., et al. 2019. Nat Chem Biol. 15: 250-258. PMID: 30643284
- Monoclonal antibodies specific for peptide epitopes of the epidermal growth factor receptor′s extracellular domain. | Baron, AT., et al. 1997. Hybridoma. 16: 259-71. PMID: 9219036
- A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera. | Baron, AT., et al. 1998. J Immunol Methods. 219: 23-43. PMID: 9831386
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A-431 Whole Cell Lysate | sc-2201 | 500 µg/200 µl | $118.00 |