SP2/0 Whole Cell Lysate: sc-364795

SP2/0 Whole Cell Lysate is derived from SP2/0-Ag14 cells, a murine myeloma cell line commonly used in hybridoma technology for the production of monoclonal antibodies. This lysate is extensively utilized in immunological research to investigate the molecular mechanisms of antibody production, B-cell signaling, and myeloma cell biology. SP2/0 cells are known for their high proliferation rate and stable secretion of immunoglobulins when fused with B-cells, making the lysate valuable for studying the expression, phosphorylation, and interactions of proteins involved in immunoglobulin synthesis and secretion. Researchers utilize SP2/0 Whole Cell Lysate to analyze key signaling pathways such as JAK/STAT, NF-κB, and PI3K/AKT using techniques like western blotting, immunoprecipitation, and mass spectrometry. This lysate is particularly useful for examining the molecular basis of B-cell receptor signaling, plasma cell differentiation, and the cellular stress responses associated with high-rate protein synthesis. Additionally, SP2/0 Whole Cell Lysate aids in screening chemical compounds that affect these signaling pathways, providing insights into the modulation of immune cell function. By comparing SP2/0 lysates with those from normal plasma cells, scientists can identify specific molecular alterations associated with myeloma cell biology, advancing research in immunology, hybridoma technology, and protein production.