PC-12 + NGF Cell Lysate: sc-3808

PC-12 + NGF cell lysate is derived from PC-12 cells, a rat pheochromocytoma cell line, treated with nerve growth factor (NGF). This lysate is extensively used in scientific research to study neuronal differentiation and the mechanisms of signal transduction. NGF treatment induces PC-12 cells to differentiate into neuron-like cells, making the lysate an invaluable resource for examining neurobiological processes. Researchers utilize PC-12 + NGF cell lysate to explore the activation of the TrkA receptor and its downstream signaling pathways, including the Ras-MAPK/ERK, PI3K-Akt, and PLCγ pathways. This lysate facilitates the study of protein phosphorylation events and the regulation of transcription factors such as CREB and NF-κB, which are crucial for neuronal survival, differentiation, and synaptic plasticity. Additionally, it is used to investigate the effects of various chemical modulators on NGF-induced signaling pathways. Recent research has leveraged this lysate for proteomic analyses to identify novel proteins and post-translational modifications associated with neuronal function and differentiation. By providing a comprehensive understanding of NGF-mediated signaling, PC-12 + NGF cell lysate continues to be a critical tool for advancing our knowledge of neurobiology and cellular signaling mechanisms in a controlled experimental environment.