C2C12 Whole Cell Lysate: sc-364188

The C2C12 Whole Cell Lysate is derived from the C2C12 mouse myoblast cell line, which originates from satellite cells of murine skeletal muscle. This lysate is a comprehensive blend of proteins, nucleic acids, lipids, and other intracellular components obtained by rupturing the C2C12 cells, commonly through methods such as sonication, detergent lysis, or enzymatic breakdown. As a tool in basic research, the C2C12 lysate serves multiple functions, primarily used by scientists to understand muscle development and differentiation. The lysate provides a snapshot of the cellular environment, offering researchers a valuable resource for studying muscle biology, gene expression, and protein interactions under various experimental conditions. It is extensively utilized in comparative proteomics to identify and quantify protein expression differences by serving as a reference or control sample. Additionally, the C2C12 lysate is crucial in validating the specificity of antibodies, as researchers use it to confirm that their antibodies are recognizing the correct proteins in muscle cells. This lysate plays a pivotal role in non-medical scientific inquiry, helping to explain the molecular mechanisms underlying muscle physiology and cellular biochemistry.

C2C12 Whole Cell Lysate References:

1. Histone deacetylase 4 associates with extracellular signal-regulated kinases 1 and 2, and its cellular localization is regulated by oncogenic Ras.  |  Zhou, X., et al. 2000. Proc Natl Acad Sci U S A. 97: 14329-33. PMID: 11114188
2. Expression of phospholipase C beta family isoenzymes in C2C12 myoblasts during terminal differentiation.  |  Faenza, I., et al. 2004. J Cell Physiol. 200: 291-6. PMID: 15174099
3. Stem cell antigen-1 is necessary for cell-cycle withdrawal and myoblast differentiation in C2C12 cells.  |  Epting, CL., et al. 2004. J Cell Sci. 117: 6185-95. PMID: 15546912
4. Regulation of TIMP-2, MT1-MMP, and MMP-2 expression during C2C12 differentiation.  |  Lluri, G. and Jaworski, DM. 2005. Muscle Nerve. 32: 492-9. PMID: 16003733
5. Subnuclear localization and differentiation-dependent increased expression of DGK-zeta in C2C12 mouse myoblasts.  |  Evangelisti, C., et al. 2006. J Cell Physiol. 209: 370-8. PMID: 16897754
6. PDZRN3 (LNX3, SEMCAP3) is required for the differentiation of C2C12 myoblasts into myotubes.  |  Ko, JA., et al. 2006. J Cell Sci. 119: 5106-13. PMID: 17118964
7. TRB3 modulates C2C12 differentiation by interfering with Akt activation.  |  Kato, S. and Du, K. 2007. Biochem Biophys Res Commun. 353: 933-8. PMID: 17207467
8. Lamin A Ser404 is a nuclear target of Akt phosphorylation in C2C12 cells.  |  Cenni, V., et al. 2008. J Proteome Res. 7: 4727-35. PMID: 18808171
9. Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue.  |  Milanesi, L., et al. 2009. Steroids. 74: 489-97. PMID: 19428437
10. The mTORC2 complex regulates terminal differentiation of C2C12 myoblasts.  |  Shu, L. and Houghton, PJ. 2009. Mol Cell Biol. 29: 4691-700. PMID: 19564418
11. Agrin induces association of Chrna1 mRNA and nicotinic acetylcholine receptor in C2C12 myotubes.  |  Chang, YF., et al. 2012. FEBS Lett. 586: 3111-6. PMID: 22884571
12. Mechanisms of insulin resistance by simvastatin in C2C12 myotubes and in mouse skeletal muscle.  |  Sanvee, GM., et al. 2019. Biochem Pharmacol. 164: 23-33. PMID: 30796916
13. Antioxidant Properties and Cytoprotective Effect of Pistacia lentiscus L. Seed Oil against 7β-Hydroxycholesterol-Induced Toxicity in C2C12 Myoblasts: Reduction in Oxidative Stress, Mitochondrial and Peroxisomal Dysfunctions and Attenuation of Cell Death.  |  Ghzaiel, I., et al. 2021. Antioxidants (Basel). 10: PMID: 34829643