3T3-L1 Cell Lysate: sc-2243
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- mouse whole cell lysate; pre-adipose embryo fibroblast cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The 3T3-L1 Cell Lysate is a crucial research tool derived from the 3T3-L1 cell line, which originates from mouse embryo fibroblasts and is renowned for its capacity to differentiate into adipocytes under appropriate conditions. This lysate is obtained through a careful process of culturing the 3T3-L1 cells, inducing adipogenesis, and subsequently lysing the cells to release their intracellular contents, which include a wide array of proteins, lipids, and other cellular components. Researchers frequently utilize the 3T3-L1 Cell Lysate to study adipocyte biology, particularly focusing on the molecular mechanisms underlying adipogenesis, lipid metabolism, and insulin signaling pathways. This lysate is instrumental in proteomics and genomics studies, facilitating detailed analyses such as Western blotting, mass spectrometry, and RNA sequencing. By providing a rich source of cellular proteins and other biomolecules, the 3T3-L1 Cell Lysate enables scientists to investigate the effects of various treatments and conditions on adipocyte function and metabolism. Additionally, it serves as a valuable reference for validating antibodies and other research reagents, contributing to the reproducibility and reliability of experimental results. Overall, the 3T3-L1 Cell Lysate plays a pivotal role in advancing our understanding of adipocyte physiology and related metabolic processes in the field of biological research.
3T3-L1 Cell Lysate References:
- Androgens decrease plasma adiponectin, an insulin-sensitizing adipocyte-derived protein. | Nishizawa, H., et al. 2002. Diabetes. 51: 2734-41. PMID: 12196466
- Nitric oxide-induced downregulation of leptin production by 3T3-L1 adipocytes. | Unno, Y., et al. 2006. Nitric Oxide. 15: 125-32. PMID: 16442319
- Leucyl/phenylalanyl(L/F)-tRNA-protein transferase-mediated aminoacyl transfer of a nonnatural amino acid to the N-terminus of peptides and proteins and subsequent functionalization by bioorthogonal reactions. | Taki, M. and Sisido, M. 2007. Biopolymers. 88: 263-71. PMID: 17216634
- Adipogenesis licensing and execution are disparately linked to cell proliferation. | Guo, W., et al. 2009. Cell Res. 19: 216-23. PMID: 19065151
- Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. | Fromm-Dornieden, C., et al. 2012. BMC Mol Biol. 13: 9. PMID: 22436005
- Anti-obesity properties of a Sasa quelpaertensis extract in high-fat diet-induced obese mice. | Kang, SI., et al. 2012. Biosci Biotechnol Biochem. 76: 755-61. PMID: 22484945
- Fisetin up-regulates the expression of adiponectin in 3T3-L1 adipocytes via the activation of silent mating type information regulation 2 homologue 1 (SIRT1)-deacetylase and peroxisome proliferator-activated receptors (PPARs). | Jin, T., et al. 2014. J Agric Food Chem. 62: 10468-74. PMID: 25286082
- An Alternative Strategy for Pan-acetyl-lysine Antibody Generation. | Kim, SY., et al. 2016. PLoS One. 11: e0162528. PMID: 27606599
- Hypolipidemic Activity of Quercus acutissima Fruit Ethanol Extract is Mediated by Inhibition of Acetylation. | Hwang, JT., et al. 2017. J Med Food. 20: 542-549. PMID: 28581876
- Knockdown of aquaporin-8 induces mitochondrial dysfunction in 3T3-L1 cells. | Ikaga, R., et al. 2015. Biochem Biophys Rep. 4: 187-195. PMID: 29124204
- Exposure of adipocytes to bisphenol-A in vitro interferes with insulin action without enhancing adipogenesis. | De Filippis, E., et al. 2018. PLoS One. 13: e0201122. PMID: 30133442
- Nod1-mediated lipolysis promotes diacylglycerol accumulation and successive inflammation via PKCδ-IRAK axis in adipocytes. | Sharma, A., et al. 2019. Biochim Biophys Acta Mol Basis Dis. 1865: 136-146. PMID: 30391544
- Development and validation of an LC-MS/MS method for tyrphostin A9. | Meyer, LF. and Shah, DK. 2019. J Pharm Anal. 9: 163-169. PMID: 31297293
- KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2α. | Pirone, L., et al. 2019. Biochim Biophys Acta Mol Cell Biol Lipids. 1864: 158514. PMID: 31465887
- Apigenin inhibits STAT3/CD36 signaling axis and reduces visceral obesity. | Su, T., et al. 2020. Pharmacol Res. 152: 104586. PMID: 31877350
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
3T3-L1 Cell Lysate | sc-2243 | 500 µg/200 µl | $118.00 |