PC-3 Cell Lysate: sc-2220

PC-3 Cell Lysate is derived from the PC-3 cell line, an androgen-independent human prostate cancer cell line commonly used in cancer biology research to study prostate cancer mechanisms and cellular responses. This cell lysate contains a rich assortment of cellular proteins, providing a snapshot of the protein expression profile under various experimental conditions, ideal for studying signal transduction, cellular proliferation, and apoptosis mechanisms in prostate cancer cells. Researchers utilize PC-3 lysate primarily for understanding the molecular pathways that drive prostate cancer progression, such as the PI3K/Akt pathway, known for its role in promoting cell survival and growth independent of androgen signaling. This lysate also serves as a valuable tool for investigating the interactions between cancer cells and the tumor microenvironment, particularly in studying how prostate cancer cells evade apoptotic signals. Further, it assists in the examination of cellular responses to hypoxia, as PC-3 cells are particularly adept at adapting to low oxygen environments, which is pivotal for understanding cancer metastasis. By providing a comprehensive proteomic landscape, PC-3 Cell Lysate enables detailed studies into the biochemistry of prostate cancer cells.

PC-3 Cell Lysate References:

1. Expression of membrane-type 1 matrix metalloproteinase (MT1-MMP) on prostate cancer cell lines.  |  Nagakawa, O., et al. 2000. Cancer Lett. 155: 173-9. PMID: 10822133
2. Cyclooxygenase-2 is up-regulated in proliferative inflammatory atrophy of the prostate, but not in prostate carcinoma.  |  Zha, S., et al. 2001. Cancer Res. 61: 8617-23. PMID: 11751373
3. Phenethyl isothiocyanate-induced apoptosis in p53-deficient PC-3 human prostate cancer cell line is mediated by extracellular signal-regulated kinases.  |  Xiao, D. and Singh, SV. 2002. Cancer Res. 62: 3615-9. PMID: 12097262
4. Downregulation of PTEN/MMAC/TEP1 expression in human prostate cancer cell line DU145 by growth stimuli.  |  Bastola, DR., et al. 2002. Mol Cell Biochem. 236: 75-81. PMID: 12190124
5. Cross-talk of anosmin-1, the protein implicated in X-linked Kallmann′s syndrome, with heparan sulphate and urokinase-type plasminogen activator.  |  Hu, Y., et al. 2004. Biochem J. 384: 495-505. PMID: 15324302
6. Guggulsterone-induced apoptosis in human prostate cancer cells is caused by reactive oxygen intermediate dependent activation of c-Jun NH2-terminal kinase.  |  Singh, SV., et al. 2007. Cancer Res. 67: 7439-49. PMID: 17671214
7. One-step isolation of plasma membrane proteins using magnetic beads with immobilized concanavalin A.  |  Lee, YC., et al. 2008. Protein Expr Purif. 62: 223-9. PMID: 18765283
8. Knockdown of protein tyrosine phosphatase SHP-1 inhibits G1/S progression in prostate cancer cells through the regulation of components of the cell-cycle machinery.  |  Rodríguez-Ubreva, FJ., et al. 2010. Oncogene. 29: 345-55. PMID: 19838216
9. Enhancement of osteoclastogenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts.  |  Shiirevnyamba, A., et al. 2011. Br J Cancer. 104: 505-13. PMID: 21206493
10. Small-molecule targeting of proliferating cell nuclear antigen chromatin association inhibits tumor cell growth.  |  Tan, Z., et al. 2012. Mol Pharmacol. 81: 811-9. PMID: 22399488
11. Rhodium(II) metallopeptide catalyst design enables fine control in selective functionalization of natural SH3 domains.  |  Vohidov, F., et al. 2015. Angew Chem Int Ed Engl. 54: 4587-91. PMID: 25688989
12. AIEgen-based theranostic system: targeted imaging of cancer cells and adjuvant amplification of antitumor efficacy of paclitaxel.  |  Chen, C., et al. 2017. Chem Sci. 8: 2191-2198. PMID: 28507673
13. 7-Farnesyloxycoumarin Exerts Anti-cancer Effects on a Prostate Cancer Cell Line by 15-LOX-1 Inhibition.  |  Saboormaleki, S., et al. 2018. Arch Iran Med. 21: 251-259. PMID: 29940744