ARPE-19 Whole Cell Lysate: sc-364357
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; retinal epithelial cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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The ARPE-19 Whole Cell Lysate is derived from the ARPE-19 cell line, a line of human retinal pigment epithelial (RPE) cells, which plays a fundamental role in retinal research. These cells were originally isolated from the retina of a 19-year-old male and have been used to study the physiology and molecular biology of the retinal pigment epithelium extensively. The ARPE-19 lysate contains a diverse array of cellular components, including proteins, lipids, nucleic acids, and other metabolites, representing the cellular environment of RPE cells. In research, this lysate facilitates the exploration of cellular processes such as phagocytosis, visual cycle, and barrier functions that are pivotal to retinal health and function. Researchers utilize ARPE-19 lysates to delve into protein expression patterns, signaling pathways, and cellular responses to oxidative stress and light exposure, which are critical in understanding retinal diseases. The lysate is particularly valuable in comparative studies where RPE responses are analyzed against various stressors or conditions, employing techniques like Western blotting, mass spectrometry, and immunoprecipitation. Such studies help explain the molecular mechanisms that underpin the RPE's response to pathological changes, providing insights that advance the fundamental understanding of retinal biology
ARPE-19 Whole Cell Lysate References:
- CNOT3 is a modifier of PRPF31 mutations in retinitis pigmentosa with incomplete penetrance. | Venturini, G., et al. 2012. PLoS Genet. 8: e1003040. PMID: 23144630
- LC-MS/MS Based Quantitation of ABC and SLC Transporter Proteins in Plasma Membranes of Cultured Primary Human Retinal Pigment Epithelium Cells and Immortalized ARPE19 Cell Line. | Pelkonen, L., et al. 2017. Mol Pharm. 14: 605-613. PMID: 28112518
- Melatonin Modulates Prohibitin and Cytoskeleton in the Retinal Pigment Epithelium. | Sripathi, SR., et al. 2017. Int J Sci Eng Res. 8: 502-506. PMID: 28845390
- ARPE-19, a human retinal pigment epithelial cell line with differentiated properties. | Dunn, KC., et al. 1996. Exp Eye Res. 62: 155-69. PMID: 8698076
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARPE-19 Whole Cell Lysate | sc-364357 | 500 µg/200 µl | $118.00 |