HL-60 + LPS Cell Lysate: sc-24704

The HL-60 + LPS Cell Lysate is derived from the HL-60 cell line, a human promyelocytic leukemia cell line established from a 36-year-old female patient. This lysate is prepared by treating HL-60 cells with lipopolysaccharide (LPS), a potent endotoxin derived from the outer membrane of Gram-negative bacteria. LPS treatment induces an inflammatory response and differentiation of HL-60 cells into macrophage-like cells, making this lysate an essential tool for studying inflammatory signaling pathways and macrophage biology. Researchers use the HL-60 + LPS Cell Lysate to investigate the molecular mechanisms underlying the inflammatory response, focusing on changes in gene expression, cytokine production, and signal transduction pathways such as NF-κB activation. This lysate is frequently utilized in proteomic analyses, Western blotting, and mass spectrometry to identify and quantify proteins involved in the inflammatory response and to understand their roles in cell signaling and function. It serves as a model system for evaluating the effects of various chemical compounds on inflammation and macrophage differentiation, aiding in the identification of key regulatory molecules and pathways. The HL-60 + LPS Cell Lysate provides a robust and reproducible platform for exploring the complex mechanisms of inflammation and advancing research in immunology and leukemic cell biology.