ECV304 Cell Lysate: sc-2269

ECV304 cell lysate, derived from a cell line originally thought to be endothelial but later identified as a contaminant from a bladder carcinoma cell line (T24), is widely utilized in studies related to cancer biology and cellular behavior. This lysate offers valuable insights into the mechanisms of angiogenesis, migration, and cellular adhesion, processes critical in cancer progression and metastasis. Researchers use ECV304 lysate to investigate the expression and activity of proteins involved in cell-to-cell interactions and migration, such as integrins and cadherins, as well as signaling pathways like Rho/ROCK and PI3K/Akt, which are fundamental in regulating cytoskeletal dynamics and cellular motility. The ability of ECV304 cells to form tubule-like structures in vitro makes the lysate particularly useful for studying pseudo-angiogenic processes. Additionally, this lysate aids in the exploration of cellular responses to hypoxic conditions, a common feature in solid tumors, providing a model to understand how cancer cells adapt to low oxygen environments. By studying these aspects, ECV304 cell lysate contributes to the broader understanding of tumor biology and cellular mechanisms of adaptation.

ECV304 Cell Lysate References:

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5. Up regulation of interleukin-8 expressions induced by mast cell tryptase via protease activated receptor-2 in endothelial cell line.  |  Lu, C., et al. 2005. Chin Med J (Engl). 118: 1900-6. PMID: 16313845
6. [Effects of Tanshinone IIA on procoagulant activity of human ECV304 cell line induced by NB4 cells].  |  Zhang, HL., et al. 2006. Sichuan Da Xue Xue Bao Yi Xue Ban. 37: 55-9. PMID: 16468642
7. The mechanism of hyperoside protection of ECV-304 cells against tert-butyl hydroperoxide-induced injury.  |  Li, HB., et al. 2008. Pharmacology. 82: 105-13. PMID: 18552516
8. Roles of small GTPase Rac1 in the regulation of actin cytoskeleton during dengue virus infection.  |  Wang, JL., et al. 2010. PLoS Negl Trop Dis. 4: PMID: 20824170
9. EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells.  |  Leng, W., et al. 2015. Int J Inflam. 2015: 301562. PMID: 26881174
10. Characterization and use of the ECV304 autoantigenic citrullinome to understand anti-citrullinated protein/peptide autoantibodies in rheumatoid arthritis.  |  de França, NR., et al. 2022. Arthritis Res Ther. 24: 23. PMID: 35027076
11. Characterization of tissue factor expression on the human endothelial cell line ECV304.  |  López-Pedrera, C., et al. 1997. Am J Hematol. 56: 71-8. PMID: 9326347
12. Dynamic activation of endothelial nitric oxide synthase by Hsp90.  |  García-Cardeña, G., et al. 1998. Nature. 392: 821-4. PMID: 9580552
13. Localization of RhoA GTPase to endothelial caveolae-enriched membrane domains.  |  Gingras, D., et al. 1998. Biochem Biophys Res Commun. 247: 888-93. PMID: 9647788