CD45 Antibody (YTH80.103) recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and FCM

CD45 Antibody (YTH80.103): sc-59071

CD45 Antibody (YTH80.103) is rated 4.0 out of 5 by 3.
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Datasheets

    • CD45 Antibody (YTH80.103) is a rat monoclonal IgG2b, cited in 3 publications, provided at 200 µg/ml
    • raised against T lymphocytes of human origin
    • recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can CD45 (YTH80.103): sc-59071 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (YTH80.103): sc-59071 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Strong band in WB in GA Strong band in WB in GA-10 whole cell lysate. -SCBT QC
Date published: 2015-05-11
Rated 4 out of 5 by from Positive band seen in WB in U Positive band seen in WB in U-698-M whole cell lysate. -SCBT QC
Date published: 2015-03-16
Rated 4 out of 5 by from Produced robust Indirect FCM data for human Produced robust Indirect FCM data for human peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2013-12-20
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