CD45 Antibody (OX30): sc-53047

CD45 Antibody (OX30) is a mouse monoclonal IgG2a kappa light chain antibody developed to recognize CD45, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45 is an essential transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in mice and rats, playing a pivotal role in regulating immune cell signaling and activation. Located on the cell membrane, CD45 modulates critical signal transduction pathways by dephosphorylating tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70. This strategic cellular positioning is crucial for controlling T and B cell receptor signaling, thereby influencing lymphocyte development, activation, and overall immune responses. Anti-CD45 antibody (OX30) is raised against lymph node glycoproteins and cells of rat origin, ensuring effective detection of CD45 in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD45 monoclonal antibody (OX30) is capable of recognizing multiple isoforms of CD45, which arise from alternative splicing of the CD45 gene, allowing for comprehensive analysis of different immune cell subsets. CD45 (OX30) antibody facilitates the study of immune cell differentiation and function, making it an invaluable tool for both research and diagnostic purposes in immunology and hematology. CD45 (OX30) monoclonal antibody enhances utility in diverse experimental settings, supporting advancements in understanding immune mechanisms and the development of targeted therapies for immune-related diseases.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD45 Antibody (OX30) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-CD45 Antibody (OX30) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD45 Antibody (OX30) Specifications

Alternate Product Name: PTPRC

Product Clone Name: OX30

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: CD45

Epitope Species: rat

Product Reactivity/Detection: a monomorphic determinant of CD45

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for CD45 Antibody (OX30): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.