CD45 Antibody (OX30) recommended for detection of a monomorphic determinant of CD45 of mouse and rat origin by WB, IP, IF, IHC(P) and FCM

CD45 Antibody (OX30): sc-53047

CD45 Antibody (OX30) is rated 4.3 out of 5 by 4.
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Datasheets

    • CD45 Antibody (OX30) is a mouse monoclonal IgG2a κ, cited in 12 publications, provided at 200 µg/ml
    • raised against lymph node glycoproteins and cells of rat origin
    • Anti-CD45 Antibody (OX30) is recommended for detection of a monomorphic determinant of CD45 of mouse and rat origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD45 Antibody (OX30) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (OX30): sc-53047.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for CD45 Antibody (OX30) for WB and IHC(P) applications. This reagent is now offered in a bundle with CD45 Antibody (OX30) (see ordering information below).

If I want to use this antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: cjMara
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 4 out of 5 by from 很好,一次成功 几个月前从朋友那借了点抗体,效果很好,没想到第一次做,就出阳性结果了!真的很开心,也非常感谢您们公司!
Date published: 2019-05-27
Rated 5 out of 5 by from Great for Flow Cytometry Positive results obtain with CD45 Antibody (OX30) by Flow cytometry as well as WB
Date published: 2017-01-17
Rated 4 out of 5 by from Single band with some background for WB in Single band with some background for WB in rat PBL whole cell lysate. -SCBT QC
Date published: 2015-04-03
Rated 4 out of 5 by from Produced good Indirect FCM data for rat peripheral Produced good Indirect FCM data for rat peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2014-12-15
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