m-IgG Fc BP-HRP mouse IgG Fc binding protein (m-IgG Fc BP) conjugated to horseradish peroxidase (HRP), for WB [ECL] and IHC(P)

m-IgG Fc BP-HRP: sc-525409

m-IgG Fc BP-HRP is rated 4.9 out of 5 by 17.
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Datasheets

    • mouse IgG Fc binding protein (m-IgG Fc BP) conjugated to Horseradish Peroxidase (HRP)
    • supplied at 50 µg in 0.5 ml volume
    • Highly recommended alternative to conventional anti-mouse IgG secondary antibodies for chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
    • Suitable for binding to the Fc region of about 53%, but not all, mouse IgG1, IgG2a and IgG2b immunoglobulins, and to a lesser extent, to mouse IgG3; not suitable for use with mouse monoclonal IgM, IgA or IgE antibodies
    • Not cross-reactive with human, rat, rabbit or goat IgG antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
    • Also see our new m-IgG Fc BP-CFL 488, CFL 555, CFL 594, CFL 647, CFL 680 and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-mouse secondary antibody fluorescent dye conjugates.
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What species is this antibody raised in? What is the best blocking method for IHC with this product?

Asked by: Nico1
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Rated 5 out of 5 by from 二抗很不错 二抗sc525409没有问题,一个多月前买的,刚用,和别的抗体不一样,这家抗体都是在4℃待着,挺方便的,不用缓化
Date published: 2022-03-12
Rated 5 out of 5 by from 显色正常 试了三个一抗,淀粉样蛋白(B-4)sc-28365条带清晰,sc-13533和sc-518035不行。
Date published: 2022-03-12
Rated 5 out of 5 by from 效果不错,很满意 客户购买的,满足实验要求,产品质量不错,表示会继续支持相关产品,如果折扣或者价格更有优势将会更有竞争力,谢谢
Date published: 2022-03-04
Rated 5 out of 5 by from Good antibody This secondary antibody allows the detection of nice, sharp bands in chemiluminescence Western blotting (WB [ECL]).
Date published: 2022-02-18
Rated 5 out of 5 by from good clear image in western blot analysis, it is good quantity.
Date published: 2021-09-13
Rated 5 out of 5 by from Good secondary antibody Our lab used this secondary antibody to probe our mouse primary antibody . It worked very well in the western blot.
Date published: 2021-07-12
Rated 5 out of 5 by from great one My lab used this antibody for detecting primary antibody from Santa Cruz. it worked very well.
Date published: 2021-07-10
Rated 5 out of 5 by from Good secondary Tested against B-Actin and GAPDH at 1:10,000 dilution and worked well for WB.
Date published: 2021-07-09
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