Anti-CD45 Antibody (35-Z6) is a mouse monoclonal IgG1 κ CD45 antibody, cited in 74 publications, provided at 200 µg/ml
raised against the extracellular domain of CD45 of human origin
Anti-CD45 Antibody (35-Z6) is recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
Anti-CD45 Antibody (35-Z6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (35-Z6): sc-1178.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD45 Antibody (35-Z6) for WB and IHC(P) applications. These reagents are now offered in bundles with CD45 Antibody (35-Z6) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CD45 Antibody (35-Z6) is a high quality monoclonal CD45 antibody (also designated PTPRC antibody or protein tyrosine phosphatase receptor type C antibody) suitable for the detection of the CD45 protein of mouse, rat and human origin. CD45 Antibody (35-Z6) is available as both the non-conjugated anti-CD45 antibody form, as well as multiple conjugated forms of anti-CD45 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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