CD45 Antibody (35-Z6): sc-1178

CD45 Antibody (35-Z6) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect CD45, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC), in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), PAR assays, and flow cytometry (FCM). Anti-CD45 antibody (35-Z6) targets the extracellular domain of human CD45, ensuring accurate recognition across multiple species. CD45 is a crucial transmembrane glycoprotein broadly expressed on hematopoietic cells, where it plays a vital role in regulating immune cell signaling. By functioning as a phosphotyrosine phosphatase, CD45 modulates the activity of Src and Syk family kinases, such as Lck, Fyn, and ZAP-70, which are essential for initiating signaling cascades following T and B cell receptor engagement. This regulation is fundamental for effective T cell activation, proliferation, and differentiation, as well as for B cell development and antibody production. The diverse isoforms of CD45, resulting from alternative splicing of exons 4, 5, and 6, allow for fine-tuned control of immune responses tailored to specific cell types and stages of activation. Dysregulation of CD45 has been implicated in various immune disorders, including autoimmune diseases and leukemias, highlighting its importance in maintaining immune homeostasis and its potential as a therapeutic target. CD45 (35-Z6) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing versatile options for a wide range of experimental applications. Anti-CD45 antibody (35-Z6) can effectively study the expression, isoform distribution, and functional dynamics of CD45, thereby advancing our understanding of immune cell biology and related disease mechanisms.

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CD45 Antibody (35-Z6) References:

1. CD45 protein-tyrosine phosphatase inhibitor development.  |  Lee, K. and Burke, TR. 2003. Curr Top Med Chem. 3: 797-807. PMID: 12678845
2. CD45: direct and indirect government of immune regulation.  |  Huntington, ND. and Tarlinton, DM. 2004. Immunol Lett. 94: 167-74. PMID: 15275963
3. CD45 glycosylation controls T-cell life and death.  |  Earl, LA. and Baum, LG. 2008. Immunol Cell Biol. 86: 608-15. PMID: 18607388
4. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression.  |  Bazil, V., et al. 1989. Immunogenetics. 29: 202-5. PMID: 2466778
5. Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.  |  Johnson, P., et al. 1989. J Exp Med. 169: 1179-84. PMID: 2466938
6. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.  |  Streuli, M., et al. 1987. J Exp Med. 166: 1548-66. PMID: 2824653
7. Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections.  |  Poppema, S., et al. 1987. Am J Pathol. 127: 418-29. PMID: 3296769
8. The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections.  |  West, KP., et al. 1986. J Pathol. 150: 89-101. PMID: 3540244
9. New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections.  |  Hall, PA., et al. 1987. J Clin Pathol. 40: 151-6. PMID: 3546393
10. Regulation of immune function by protein tyrosine phosphatases.  |  Okumura, M. and Thomas, ML. 1995. Curr Opin Immunol. 7: 312-9. PMID: 7546394
11. Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200.  |  Trowbridge, IS. 1978. J Exp Med. 148: 313-23. PMID: 78961
12. CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development.  |  Trowbridge, IS. and Thomas, ML. 1994. Annu Rev Immunol. 12: 85-116. PMID: 8011300

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD45 Antibody (35-Z6) has 98 citations in a variety of scientific publications.

Primary antibodies like Anti-CD45 Antibody (35-Z6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD45 Antibody (35-Z6) Specifications

Alternate Product Name: PTPRC

Product Clone Name: 35-Z6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD45

Epitope Species: human

Product Reactivity/Detection: CD45

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 98 citations in various scientific publications

Available Conjugates/Forms for CD45 Antibody (35-Z6): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin