CD45 Antibody (35-Z6) recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

CD45 Antibody (35-Z6): sc-1178

CD45 Antibody (35-Z6) is rated 4.3 out of 5 by 26.
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Datasheets

    • Anti-CD45 Antibody (35-Z6) is a mouse monoclonal IgG1 (kappa light chain) CD45 antibody provided at 200 µg/ml
    • raised against the extracellular domain of CD45 of human origin
    • Anti-CD45 Antibody (35-Z6) is recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD45 Antibody (35-Z6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD45 (35-Z6): sc-1178.

If I am going to stain mouse skin tissues, do I need additional kit, for example "mouse on mouse kit" (as this antibody is mouse origin)?

Asked by: hpark
Thank you for your question. It depends on what type of tissues you are staining. If you are staining mouse skin tissues, and there is not a lot of endogenous mouse IgG, then it is probably not necessary to use a special "mouse on mouse kit" for staining. We do recommend including a negative control tissue where you omit primary antibody and stain with secondary antibody alone. Then you can identify any possible non-specific binding from your anti-mouse IgG secondary antibody alone. As an alternative, you could also consider using one of the directly conjugated forms available of this CD45 antibody. Then no secondary antibody is needed for detection, and you can avoid any possible problem of non-specific binding from anti-mouse IgG secondary antibodies.
Answered by: Technical Support
Date published: 2020-05-18

What could be the expiry date for CD45 since we have observed it to be active even after 5 years

Asked by: Samarth
Thank you for your question. We do not give our antibodies an expiration date since they can work for up to 10-15 years in some cases. We warranty the performance of our antibody reagents for 1 year from the date of purchase.
Answered by: Tech Service
Date published: 2020-02-26

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CD45 Antibody (35-Z6): sc-1178?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 5 out of 5 by from Great CD45 Ab for DAB immunohistochemistry The Ab gives strong and clean signal/noise staining of CD45 in FFPE colon tissue from mouse
Date published: 2019-09-20
Rated 5 out of 5 by from immunofluorescent staining on immunofluorescent staining this sc-1178 CD45 antibody worked well.
Date published: 2019-04-16
Rated 4 out of 5 by from Consistent Quality We continue to use this antibody for IF and flow, though have not had success with IP.
Date published: 2019-03-18
Rated 1 out of 5 by from Bad result on mouse samples Bad result on mouse bone marrow paraffin samples. Few cells stained.
Date published: 2018-03-15
Rated 4 out of 5 by from Good for Rat and Mouse Received this as a sample. We ran 3 species (H, M, R) on 3 separate test runs. HIER at high pH 9.0 is necessary. The mouse and rat spleens show strong results. The human tissues were very weak, tried both normal and abnormal tissues with same results. We used a concentration of 1:100. If you are running human tissue, I would suggest using a higher concentration than ours.
Date published: 2017-12-20
Rated 4 out of 5 by from Overall Great! Antibody worked well for IF staining of human T cells at a 1:100 dilution. Also good for flow at 1ug/1 million cells. Western blot seemed good/relatively clean. Did not work for IP at a concentration of 1ug/IP.
Date published: 2017-11-20
Rated 4 out of 5 by from mouse anti-CD45 antibody I got a free sample and tried on IHC-P. It worked ok, but not very strong.
Date published: 2017-09-21
Rated 5 out of 5 by from flow cytometry The CD45 in lymphocytes was detected by flow cytometry. We obtained specific staining and 1:300 dilution used.
Date published: 2017-09-16
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