CD45RA Antibody (F8-11-13): sc-19665

CD45RA Antibody (F8-11-13) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target CD45 protein, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45 is a vital transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in humans and monkeys, playing a crucial role in regulating immune cell signaling and activation. Structurally, CD45 comprises an extracellular domain with multiple isoforms generated through alternative splicing of exons 4, 5, and 6, a single transmembrane region, and a conserved intracellular domain that possesses phosphatase activity. This structural organization is essential for CD45′s function, as the variability in the extracellular domain allows for differential interactions with other cell surface molecules, thereby modulating signal transduction pathways critical for T and B cell receptor signaling. Located on the cell membrane, CD45 dephosphorylates key tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70, which are pivotal for initiating and sustaining signaling cascades that drive lymphocyte development, activation, and immune responses. Additionally, CD45 undergoes post-translational modifications, including extensive glycosylation, which are vital for stability, proper localization on the cell membrane, and interactions with other signaling proteins, thereby enhancing regulatory functions. Anti-CD45RA antibody (F8-11-13) is raised against T lymphocytes of human origin and is effective in various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). By binding to CD45, CD45RA monoclonal antibody (F8-11-13) facilitates comprehensive analysis of immune cell populations and their activation states, providing valuable insights into immune system dynamics and the mechanisms underlying immune-related diseases. CD45RA (F8-11-13) antibody′s ability to detect multiple isoforms of CD45, resulting from alternative splicing, allows researchers to investigate the diversity and function of different immune cell subsets, making anti-CD45RA antibody (F8-11-13) an indispensable tool for both research and diagnostic purposes in immunology and hematology.

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.