m-IgG kappa BP-HRP mouse IgG kappa binding protein (m-IgGκ BP) conjugated to horseradish peroxidase (HRP), for WB, IHC(P) and ELISA

m-IgGκ BP-HRP: sc-516102

m-IgGκ BP-HRP is rated 4.7 out of 5 by 330.
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Datasheets

    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP)
    • supplied at 200 µg in 0.5 ml volume
    • Highly recommended alternative to conventional anti-mouse IgG secondary antibodies for chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
    • Suitable for binding to most, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For a Cruz Marker™ compatible mouse IgG kappa binding protein, use m-IgGκ BP-HRP (Cruz Marker) (sc-516102-CM) (Click here for datasheet)
    • For mouse IgGλ immunoglobulins we recommend m-IgGλ BP-HRP (sc-516132)
    • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
    • Also see our new m-IgGκ BP-FITC, PE, CFL 488, CFL 555, CFL 594, CFL 647, CFL 680 and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-mouse secondary antibody fluorescent dye conjugates.
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Good afternoon, I would like to obtain CoA of this product: ref sc-516102, lot# A0521 Please let me know how can we obtain this. Thank you in advance.

Asked by: Facundo
Thank you for your question. We do not issue CoA documentation for this product. If you require any information in particular, please refer to the datasheet available on this page or reach out to our technical service team directly.
Answered by: Tech Support Europe
Date published: 2022-11-30

"GFP Antibody (B-2): sc-9996" / "m-IgGκ BP-HRP: sc-516102" bundle seems not to work to detect GFP. Any possible reason?

Asked by: YD Sun
Thank you for your question. Please contact technical service to trouble shoot at (800)-457-3801 or email [email protected]
Answered by: BlakeJ
Date published: 2022-11-17

Hello! What serum should I use for blocking of this antibody, which I am using with a horse anti-mouse primary in horse tissue?

Asked by: Hannah Kem
Thank you for your question. Our recommended blocking reagent that we use for testing of all of our antibody products is UltraCruz® Blocking Reagent: sc-516214.
Answered by: Tech Support Europe
Date published: 2022-09-05

Can this antibody be used for ELISA and what is the dilution range for ELISA?

Asked by: Priyanga
Thank you for your question. m-IgGκ BP-HRP: sc-516102 can be used to detect primary mouse IgG antibodies with a kappa light chain in ELISA applications. Optimal dilution for your particular experimental setup should be determined by titration.
Answered by: Tech Support Europe
Date published: 2022-09-05

What is the suggested incubation time and temperature when used for Immunohistochemsitry (1:50 dilution)?

Asked by: KSRP
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Anonymous
Date published: 2022-07-03

what's the difference between sc-516102 and sc-516102-cm?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2022-08-24

Will this antibody work with DAB detection in western blot?

Asked by: Ankita v
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-12-30

I have two vials of this product on hand. Unfortunately, I did not instruct my associates to mark when they were opened or when they were received. Can you tell me which vial is oldest by the lot #? I have one vial of Lot# G0617 and one of Lot# B2620.

Asked by: PapaMouse
Thank you for your question. For production dates, please contact Technical Support by phone 1800-457-3801 option 2, by email [email protected] or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Blake J
Date published: 2022-08-27
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Rated 5 out of 5 by from 功能很出色 实验以来一直用的此产品,挺好用的,尚未发现有何问题。按说明书的浓度即可,在较低浓度时也能起到较好的作用。
Date published: 2022-10-26
Rated 5 out of 5 by from 很不错 因为受到许多人的推荐,科研工作人员也在相当多的研究中使用了它,所以非常期待地选择了这款抗体,结果不负所望。
Date published: 2022-09-22
Rated 5 out of 5 by from 二抗很好用 已多次购买santa cruz的这个抗小鼠抗体的二抗产品,做western的效果很好,背景干净,条带清晰。
Date published: 2022-08-18
Rated 5 out of 5 by from 抗体很好用 之前购买了这只二抗,用于免疫印迹,以1:5000比例稀释,显色效果不错,条带非常清晰,特异性好,非常好用。
Date published: 2022-08-16
Rated 5 out of 5 by from 效果好 用于WB实验,常规ECL发光液显色。以1:5000的比例使用,效果非常好。此外,无杂带,我们会再次购买的。
Date published: 2022-08-14
Rated 5 out of 5 by from 抗体好用,已经是二次购买了 santa cruz家的抗体就是好用,这已经我们实验室第二次购买了,我们实验室的二抗都用的snata家的。
Date published: 2022-08-13
Rated 5 out of 5 by from Really good in western blot! We got a small vial of this m-IgGκ BP-HRP (sc-516102) a few days ago, and used it in western blot as alternative to conventional goat anti-mouse IgG secondary antibodies. According to the western blot imaging, we noticed its strong signal and minimal background, which lead to the scientific and reliable result. So it really works good in western blot! We know that it can be used as anti-mouse IgG secondary antibodies for immunohistochemical (IHC) signal enhancement as well. We will try to use like that next time!
Date published: 2022-08-13
Rated 5 out of 5 by from Western Blot Mouse IgG Binding Protein (No. sc-516102) is a perfect substitute for secondary antibodies, capable of specifically binding primary antibodies with good specificity.
Date published: 2022-08-13
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