mouse IgG lambda binding protein (m-IgGλ BP) conjugated to Horseradish Peroxidase (HRP)
supplied at 200 µg in 0.5 ml volume
Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
Suitable for binding to mouse IgGλ light chain immunoglobulins comprising approximately 2% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGκ light chain antibodies
Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
For a Cruz Marker™ compatible mouse IgG lambda binding protein, use m-IgGλ BP-HRP (Cruz Marker) (sc-516132-CM) (Click here for datasheet)
For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-HRP (sc-516102)
If you have an interest in testing a 10 µg sample of m-IgGλ BP-HRP (sc-516132) or m-IgGλ BP-HRP (Cruz Marker) (sc-516132-CM), our highly recommended recombinant alternatives to conventional secondary anti-mouse IgG HRP reagents, please contact our Technical Service Department (or your local Distributor) for more information.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Mouse IgGλ light chain binding protein (m-IgGλ BP) conjugated to horseradish peroxidase (HRP) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB) and Immunohistochemical (IHC) signal enhancement. Mouse IgGλ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgGλ light chain binding protein (m-IgGλ BP) is suitable for binding to mouse IgGλ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGκ light chain primary antibodies.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
We didn't know our primary antibody subtype ( kappaor lamda), which 2nd we should order?
Asked by: yuhong
Thank you for your question. The significant majority of mouse monoclonal IgG primary antibodies (98%) have a kappa light chain. And therefore we would recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP): sc-516102 for Western blotting or immunohistochemistry.
Only approximately 2% of mouse IgG contains a lambda light chain, but for those subtypes we do offer a lambda-specific mouse IgG binding protein, m-IgGλ BP-HRP: sc-516132.
Answered by: Technical Support
Date published: 2018-09-13
I was looking for an anti-rat secondary antibody to be used in WB, but I couldn´t find it. I need the secondary antibody for the primary antibodies: sc-58605, sc-58607, and sc-58596. Can you give me an alternative?
Asked by: Marcia1
Thank you for your question. Unfortunately, we do not currently offer any secondary antibodies specific for detection of rat immunoglobulins. We are working on developing new products, but these are not yet available. We recommend using any conventional anti-rat IgG secondary antibodies for detection of the primary antibodies you have referenced above.