CD45 Antibody (351C5): sc-53201
- CD45 Antibody (351C5) is a mouse monoclonal IgM κ provided at 200 µg/ml
- raised against Raji cells of human origin
- recommended for detection of CD45 of human origin by WB, IP, IF, IHC(P) and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45 Antibody (351C5). This work is in progress.
CD45 Antibody (351C5) is a mouse monoclonal IgM kappa light chain antibody engineered to target CD45, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45 is a vital transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in humans, playing a crucial role in regulating immune cell signaling and activation. Situated on the cell membrane, CD45 is essential for modulating signal transduction pathways by dephosphorylating key tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70. These interactions are fundamental for proper T and B cell receptor signaling, influencing lymphocyte development, activation, and overall immune responses. CD45 undergoes significant post-translational modifications, including extensive glycosylation, which are critical for stability, localization, and interaction with other signaling molecules, thereby enhancing phosphatase activity and regulatory functions. Anti-CD45 antibody (351C5) is raised against Raji cells of human origin, ensuring effective detection of CD45 in various human applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD45 monoclonal antibody (351C5) is capable of recognizing multiple isoforms of CD45, which result from alternative splicing of the CD45 gene, allowing for the identification and analysis of different immune cell subsets. Anti-CD45 antibody (351C5) facilitates comprehensive studies of immune cell populations and their signaling mechanisms, making this antibody an indispensable tool for both research and diagnostic purposes in immunology and hematology.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD45 Antibody (351C5) | sc-53201 | 200 µg/ml | $316.00 | |||
CD45 Antibody (351C5) FITC | sc-53201 FITC | 200 µg/ml | $330.00 | |||
CD45 Antibody (351C5) PE | sc-53201 PE | 200 µg/ml | $343.00 |